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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-30835 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COLEC12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Raji, K562. Predicted reactivity: Mouse (92%), Rat (91%), Chicken (83%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references An MRI radiomics approach to predict survival and tumour-infiltrating macrophages in gliomas.
Li G, Li L, Li Y, Qian Z, Wu F, He Y, Jiang H, Li R, Wang D, Zhai Y, Wang Z, Jiang T, Zhang J, Zhang W
Brain : a journal of neurology 2022 Apr 29;145(3):1151-1161
Brain : a journal of neurology 2022 Apr 29;145(3):1151-1161
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using COLEC12 Polyclonal Antibody (Product # PA5-30835). Sample (30 µg of whole cell lysate). Lane A: Raji. Lane B: K562 . 5% SDS PAGE. COLEC12 Polyclonal Antibody (Product # PA5-30835) diluted at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of COLEC12 was performed in paraffin-embedded rat brain tissue using COLEC12 Polyclonal Antibody (Product # PA5-30835) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Colec12 (Product # PA5-30835) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Experimental validation of RF-related tumour macrophage infiltration. ( A ) Scheme of the experimental workflow. ( B ) t -Distributed stochastic neighbour embedding (tSNE) plot shows clustering of each patient's cells based on gene expression. Point coordinates are based on tSNE dimensionality reduction of the top principal components calculated from the 5000 most informative genes. Cell colour specifies assignment of cells to these clusters inferred using shared nearest neighbour clustering. Pie charts demonstrate the distribution of the identified cell types across samples in each patient and histograms show the macrophage cell abundance between high-risk and low-risk patients. ( C ) Immunohistochemical staining displays the RF-related macrophage markers MS4A4A, STAB1 and COLEC12. The scatter diagram shows the expression level of these markers in high-risk and low-risk samples. Kaplan-Meier survival analysis was performed between the samples with high and low expression of macrophage markers.
