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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-18212 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MUNC18 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse brain (Lane 1), Rat brain (Lane 2), Rat Liver (Lane 3) and Rat Kidney (Lane 4). The blot was probed with Anti-MUNC18 Polyclonal Antibody (Product # PA5-18212, 1:2000 dilution) and detected by chemiluminescence using Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27014, 0.25 µg/mL, 1:4000 dilution). A 67 kDa band corresponding to MUNC18 was observed in the Mouse and Rat brain, while it was not detected in Rat Liver and Kidney which are reported negative for MUNC18 expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Brain lysate using Product # PA5-18212 at a concentration of 0.1 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemichal analysis of MUNC18 in Human Cortex using a MUNC18 polyclonal antibody (Product # PA5-18212) at a concentration of 5 µg/mL. The sample was paraffin embedded, and heat treated antigen retrieval was used to detect the target with AP staining.
