PA5-30184

antibody from Invitrogen Antibodies

Targeting: STXBP1 hUNC18, MUNC18-1, rbSec1, UNC18

Antibody data

Submit

Validation data

Product number: PA5-30184 - Provider product page
Provider: Invitrogen Antibodies
Product name: MUNC18 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A431, mouse brain, rat brain. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (93%), Chicken (98%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.96 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

STXBP1 protein structure - PA5-30184 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of STXBP1 using 20 µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a STXBP1 polyclonal antibody (Product # PA5-30184) at a dilution of 1:10,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extract (30 µg lysate) of Mouse brain (Lane 1), Rat brain (Lane 2), Rat Liver (Lane 3), and Rat Kidney (Lane 4). The blot was probed with Anti-MUNC18 Polyclonal Antibody (Product # PA5-30184, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 67 kDa band corresponding to MUNC18 was observed in the Mouse and Rat brain, while it was not detected in Rat Liver and Kidney which are reported negative for MUNC18 expression.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of MUNC18 was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a MUNC18 Polyclonal Antibody (Product # PA5-30184) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using MUNC18 Polyclonal Antibody (Product # PA5-30184). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431 . 7.5% SDS PAGE. MUNC18 Polyclonal Antibody (Product # PA5-30184) diluted at 1:3,000.