PA5-97202

antibody from Invitrogen Antibodies

Targeting: METTL4 FLJ23017, HsT661

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-97202

Provider

Invitrogen Antibodies

Product name

METTL4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MSVVHQLSAG WLLDHLSFIN KINYQLHQHH EPCCRKKEFT TSVHFESLQM DSVSSSGVCA AFIASDSSTK PENDDGGNYE MFTRKFVFRP ELFDVTKPYI TPAVHKECQQ SNEKEDLMNG VKKEISISII GKKRKRCVVF NQGELDAMEY HTKIRELILD GSLQLIQEGL KSGFLYPLFE KQDKGSKPIT; Positive Samples: A-549, 293T, HeLa, MCF7, Mouse brain, Rat brain

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.62 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of METTL4 in extracts of various cell lines. Samples were incubated with METTL4 Polyclonal antibody (Product # PA5-97202) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of METTL4 in NIH/3T3 cells. Samples were incubated with METTL4 Polyclonal antibody (Product # PA5-97202) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of METTL4 in U2OS cells. Samples were incubated with METTL4 Polyclonal antibody (Product # PA5-97202) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of METTL4 in paraffin-embedded human lung cancer. Samples were incubated with METTL4 Polyclonal antibody (Product # PA5-97202) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of METTL4 in paraffin-embedded mouse kidney. Samples were incubated with METTL4 Polyclonal antibody (Product # PA5-97202) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of METTL4 in paraffin-embedded rat testis. Samples were incubated with METTL4 Polyclonal antibody (Product # PA5-97202) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of METTL4 in paraffin-embedded mouse kidney. Samples were incubated with METTL4 Polyclonal antibody (Product # PA5-97202) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.