PA5-119751

antibody from Invitrogen Antibodies

Targeting: DLGAP1 DAP-1, GKAP, SAPAP1

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-119751 - Provider product page
Provider: Invitrogen Antibodies
Product name: SAPAP1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Positive Control: Human brain tissue cell lysates, PC-12, N2A, SHG-44, rat testis tissue, rat brain tissue, mouse testis tissue, mouse brain tissue, Daudi. Subcellular Location: Cell junction, Cell membrane, Membrane, Synapse.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

DLGAP1 protein structure - PA5-119751 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of of GKAP in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with SAPAP1 Polyclonal Antibody (Product # PA5-119751) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of of GKAP in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with SAPAP1 Polyclonal Antibody (Product # PA5-119751) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of of GKAP in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with SAPAP1 Polyclonal Antibody (Product # PA5-119751) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).