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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- PA5-27528 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EPB41L3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: PC-3, U87-MG. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (86%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.97 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EPB41L3 showing staining in the cytoplasm of HepG2 cells. HepG2 cells were fixed in ice-cold MeOH for 5 min and stained using an EPB41L3 polyclonal antibody (Product # PA5-27528) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EPB41L3 Polyclonal Antibody detects EPB41L3 protein at cell membrane by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EPB41L3 stained by EPB41L3 Polyclonal Antibody (Product # PA5-27528) diluted at 1:1,000. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EPB41L3 Polyclonal Antibody detects EPB41L3 protein at cell membrane by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EPB41L3 stained by EPB41L3 Polyclonal Antibody (Product # PA5-27528) diluted at 1:1,000. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using EPB41L3 (Product # PA5-27528) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
