Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27528 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EPB41L3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: PC-3, U87-MG. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (86%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.97 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EPB41L3 using A) 30 µg PC-3 whole cell lysate and B) 30 µg U87-MG whole cell lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with an EPB41L3 polyclonal antibody (Product # PA5-27528) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of EPB41L3 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a EPB41L3 Polyclonal Antibody (Product # PA5-27528) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EPB41L3 was performed by separating 30 µg of whole cell extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a EPB41L3 Polyclonal Antibody (Product # PA5-27528) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EPB41L3 showing staining in the cytoplasm of HepG2 cells. HepG2 cells were fixed in ice-cold MeOH for 5 min and stained using an EPB41L3 polyclonal antibody (Product # PA5-27528) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EPB41L3 Polyclonal Antibody detects EPB41L3 protein at cell membrane by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EPB41L3 stained by EPB41L3 Polyclonal Antibody (Product # PA5-27528) diluted at 1:1,000. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using EPB41L3 (Product # PA5-27528) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
