LS-C331290

antibody from LSBio

Targeting: RALBP1 RIP, RIP1, RLIP76

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C331290

Provider

LSBio

Product name

RIP1 / RALBP1 Antibody LS-C331290

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using RALBP1 antibody at 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of extracts from normal (control) and RALBP1 knockout (KO) HeLa cells, using RALBP1 antibodyat 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat spleen using RALBP1 antibodyat dilution of 1:100 (40x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat heart using RALBP1 antibodyat dilution of 1:100 (40x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat spleen using RALBP1 antibodyat dilution of 1:100 (40x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat heart using RALBP1 antibodyat dilution of 1:100 (40x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat spleen using RALBP1 antibodyat dilution of 1:100 (40x lens).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat heart using RALBP1 antibodyat dilution of 1:100 (40x lens).