Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB2839 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal Calcineurin A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat Calcineurin A.
- Reactivity
- Human, Mouse, Rat
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Insulin dysfunction induces in vivo tau hyperphosphorylation through distinct mechanisms.
Planel E, Tatebayashi Y, Miyasaka T, Liu L, Wang L, Herman M, Yu WH, Luchsinger JA, Wadzinski B, Duff KE, Takashima A
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Dec 12;27(50):13635-48
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Dec 12;27(50):13635-48
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat Calcineurin A by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, TS1 mouse helper T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat Calcineurin A Monoclonal Antibody (Catalog # MAB2839) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human Calcineurin (Catalog # 3160-CA) (1 ng) was included. A specific band for Calcineurin A was detected at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.