PA5-17446

antibody from Invitrogen Antibodies

Targeting: PPP3CA CALN, CALNA, CNA1, PPP2B

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17446

Provider

Invitrogen Antibodies

Product name

Calcineurin A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with protein phosphatase 1 or 2A.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

10 µg/mL

Storage

-20°C

References

Submitted references

RedoxiFluor: A microplate technique to quantify target-specific protein thiol redox state in relative percentage and molar terms.

Tuncay A, Noble A, Guille M, Cobley JN
Free radical biology & medicine 2022 Mar;181:118-129

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Nicotinamide Treatment Facilitates Mitochondrial Fission through Drp1 Activation Mediated by SIRT1-Induced Changes in Cellular Levels of cAMP and Ca(2).

Song SB, Park JS, Jang SY, Hwang ES
Cells 2021 Mar 10;10(3)

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Knockout of p21-activated kinase-1 attenuates exercise-induced cardiac remodelling through altered calcineurin signalling.

Davis RT 3rd, Simon JN, Utter M, Mungai P, Alvarez MG, Chowdhury SA, Heydemann A, Ke Y, Wolska BM, Solaro RJ
Cardiovascular research 2015 Dec 1;108(3):335-47

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Pan-Calcineurin A in Hela cells using a Pan-Calcineurin A polyclonal antibody (Product # PA5-17446) (green) showing cytoplasmic localization. Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Pan-Calcineurin A in Hela cells using a Pan-Calcineurin A polyclonal antibody (Product # PA5-17446) (green) showing cytoplasmic localization. Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 NAM-induced mitochondrial fragmentation is in part driven by calcineurin activation through an increase of Ca 2+ that is dependent on Drp1. ( A , B ) Human fibroblasts cultivated with 5 mM NAM for 24 h with 2 muM FK506 for 1 h or 20 muM forskolin for 1 h prior to harvest were lysed and applied to immunoblotting analysis with antibodies against phosphor-Drp1 or Drp1 ( A ). Mitochondria were immuno-stained with Tom20 antibody and visualized by confocal microscopy ( B ). Nuclei were counter-stained with DAPI (blue). Length of mitochondria in at least 20 cells was measured and averaged, and mean values normalized by that of control cells were plotted in the bar graph. ## indicates a significant difference ( p < 0.01) between the measured means of the cells untreated and treated with forskolin. ( C ) Cells transfected with siNeg or siCalcineurin RNA were incubated with 5 mM NAM for the indicated period, and then applied to immunoblotting analysis with antibodies against calcineurin, phosphor-Drp1, and Drp1. In ( A , C ), protein bands were quantitated, and the relative values of pDrp1 over Drp1 were normalized by those of control cells. Mean values of three different experiments were plotted and presented in bar graphs below. ( p < 0.05) between NAM''s effects in the presence and absence of forskolin ( A ) or calcineurin ( C ). ( D ) Cells transfected with siNeg RNA or siDrp1 RNA were cultured in the presence of 5 mM NAM for 48 h, 1 muM SRT1720 for 24 h, or 2muM ionomycin