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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Other assay [2]
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- Product number
- PA5-17446 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calcineurin A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody. This antibody is not cross-reactive with protein phosphatase 1 or 2A.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 10 µg/mL
- Storage
- -20°C
Submitted references RedoxiFluor: A microplate technique to quantify target-specific protein thiol redox state in relative percentage and molar terms.
Nicotinamide Treatment Facilitates Mitochondrial Fission through Drp1 Activation Mediated by SIRT1-Induced Changes in Cellular Levels of cAMP and Ca(2).
Knockout of p21-activated kinase-1 attenuates exercise-induced cardiac remodelling through altered calcineurin signalling.
Tuncay A, Noble A, Guille M, Cobley JN
Free radical biology & medicine 2022 Mar;181:118-129
Free radical biology & medicine 2022 Mar;181:118-129
Nicotinamide Treatment Facilitates Mitochondrial Fission through Drp1 Activation Mediated by SIRT1-Induced Changes in Cellular Levels of cAMP and Ca(2).
Song SB, Park JS, Jang SY, Hwang ES
Cells 2021 Mar 10;10(3)
Cells 2021 Mar 10;10(3)
Knockout of p21-activated kinase-1 attenuates exercise-induced cardiac remodelling through altered calcineurin signalling.
Davis RT 3rd, Simon JN, Utter M, Mungai P, Alvarez MG, Chowdhury SA, Heydemann A, Ke Y, Wolska BM, Solaro RJ
Cardiovascular research 2015 Dec 1;108(3):335-47
Cardiovascular research 2015 Dec 1;108(3):335-47
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of SK-N-AS (Lane 1), HT-29 (Lane 2), MCF7 (Lane 3), NIH/3T3 (Lane 4), SH-SY5Y (Lane 5), A549 (Lane 6) and tissue extract of Rat Brain (Lane 7). The blot was probed with Anti-Calcineurin A Polyclonal Antibody (Product # PA5-17446, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 59 kDa band corresponding to Calcineurin A was observed across the cell lines and tissue tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Calcineurin A was performed by loading 30 ug of HEK-293 control (Lane 1), HEK-293- Calcineurin A knockout (Lane 2) edigene lysates. The blot was probed with Calcineurin A Antibody (Product # PA5-17446, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). Loss of signal upon CRISPR mediated knockout (KO) confirms that antibody is specific to Calcineurin A. (Band at ~27kDa corresponds to the fragment of Calcineurin A).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Pan-Calcineurin A in lysates from MCF7 and NIH/3T3 cells using Pan-Calcineurin A polyclonal antibody (Product # PA5-17446).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Pan-Calcineurin A in Hela cells using a Pan-Calcineurin A polyclonal antibody (Product # PA5-17446) (green) showing cytoplasmic localization. Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Pan-Calcineurin A in Hela cells using a Pan-Calcineurin A polyclonal antibody (Product # PA5-17446) (green) showing cytoplasmic localization. Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 NAM-induced mitochondrial fragmentation is in part driven by calcineurin activation through an increase of Ca 2+ that is dependent on Drp1. ( A , B ) Human fibroblasts cultivated with 5 mM NAM for 24 h with 2 muM FK506 for 1 h or 20 muM forskolin for 1 h prior to harvest were lysed and applied to immunoblotting analysis with antibodies against phosphor-Drp1 or Drp1 ( A ). Mitochondria were immuno-stained with Tom20 antibody and visualized by confocal microscopy ( B ). Nuclei were counter-stained with DAPI (blue). Length of mitochondria in at least 20 cells was measured and averaged, and mean values normalized by that of control cells were plotted in the bar graph. ## indicates a significant difference ( p < 0.01) between the measured means of the cells untreated and treated with forskolin. ( C ) Cells transfected with siNeg or siCalcineurin RNA were incubated with 5 mM NAM for the indicated period, and then applied to immunoblotting analysis with antibodies against calcineurin, phosphor-Drp1, and Drp1. In ( A , C ), protein bands were quantitated, and the relative values of pDrp1 over Drp1 were normalized by those of control cells. Mean values of three different experiments were plotted and presented in bar graphs below. ( p < 0.05) between NAM''s effects in the presence and absence of forskolin ( A ) or calcineurin ( C ). ( D ) Cells transfected with siNeg RNA or siDrp1 RNA were cultured in the presence of 5 mM NAM for 48 h, 1 muM SRT1720 for 24 h, or 2muM ionomycin
