PA5-29209

antibody from Invitrogen Antibodies

Targeting: PPP3CA CALN, CALNA, CNA1, PPP2B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29209

Provider

Invitrogen Antibodies

Product name

Calcineurin A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, Molt-4, U87-MG, SK-N-SH, SK-N-AS, IMR32, JC, BCL-1, rat brain, human Calcineurin A-transfected 293T. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (90%), Xenopus laevis (98%), Dog (100%), Pig (99%), Rabbit (100%), Rhesus Monkey (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.98 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Calcineurin A Polyclonal Antibody detects Calcineurin A protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A Polyclonal Antibody (Product # PA5-29209) diluted by 1:3,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Calcineurin A was performed by separating 30 µg of non-transfected (-) and Calcineurin A-transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Calcineurin A Polyclonal Antibody (Product # PA5-29209) at a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Calcineurin A Polyclonal Antibody detects Calcineurin A protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10 % SDS-PAGE, and the membrane was blotted with Calcineurin A Polyclonal Antibody (Product # PA5-29209) diluted by 1:3,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Calcineurin A Polyclonal Antibody detects Calcineurin A protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A Polyclonal Antibody (Product # PA5-29209) diluted by 1:3,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Calcineurin A Polyclonal Antibody detects Calcineurin A protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A Polyclonal Antibody (Product # PA5-29209) diluted by 1:3,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Calcineurin A Polyclonal Antibody detects Calcineurin A protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5 % SDS-PAGE, and blotted with Calcineurin A Polyclonal Antibody (Product # PA5-29209) diluted by 1:3,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Calcineurin A was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcineurin A Polyclonal Antibody (Product # PA5 29209) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin immunoprecipitation analysis of Calcineurin A was performed using cross-linked chromatin from 1x10^6 HCT116 colon carcinoma cells treated with serum for 0, 15, 30, and 60 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0 µL/100 µL well volume of a Calcineurin A polyclonal antibody (Product # PA5-29209). Chromatin aliquots from ~1 x 105 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1 µL of eluted DNA in 2 µL SYBR real-time PCR reactions containing primers to amplify -15kb upstream of the Egr1 gene or exon-1 of Egr1. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the Egr-1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.