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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-29255 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calcineurin A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: human PPP3CA-transfected 293T, mouse brain, Rat brain. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (93%), Xenopus laevis (98%), Dog (100%), Pig (99%), Rabbit (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.77 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references AKT inhibition-mediated dephosphorylation of TFE3 promotes overactive autophagy independent of MTORC1 in cadmium-exposed bone mesenchymal stem cells.
Pi H, Li M, Zou L, Yang M, Deng P, Fan T, Liu M, Tian L, Tu M, Xie J, Chen M, Li H, Xi Y, Zhang L, He M, Lu Y, Chen C, Zhang T, Wang Z, Yu Z, Gao F, Zhou Z
Autophagy 2019 Apr;15(4):565-582
Autophagy 2019 Apr;15(4):565-582
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Calcineurin an in Non-transfected (-) and Calcineurin A-transfected (+) 293T whole cell extracts (30 µg). Samples were separated by 10% SDS-PAGE and the membrane was probed with Calcineurin A Polyclonal antibody (Product # PA5-29255) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Calcineurin A was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Calcineurin A Polyclonal Antibody (Product # PA5-29255) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Calcineurin A was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Calcineurin A Polyclonal Antibody (Product # PA5-29255) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Calcineurin A Polyclonal Antibody detects Calcineurin A protein by Western blot analysis. Non-transfected (-) and Calcineurin A-transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A Polyclonal Antibody (Product # PA5-29255) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Calcineurin A Polyclonal Antibody (Product # PA5-29255). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A Polyclonal Antibody (Product # PA5-29255) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Calcineurin A Rabbit Polyclonal Antibody (Product # PA5-29255) and 32 kDa, 58 kDa bands corresponding to Calcineurin A were observed across tissues tested except Mouse Thymus which is reported to be negative. Whole cell extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2) and Mouse Thymus (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Calcineurin A was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcineurin A Polyclonal Antibody (Product # PA5 29255) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Calcineurin A Polyclonal Antibody detects Calcineurin A protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Calcineurin A Polyclonal Antibody (Product # PA5-29255) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
