NB110-40553

antibody from Novus Biologicals

Targeting: PPP3CA CALN, CALNA, CNA1, PPP2B

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB110-40553

Provider

Novus Biologicals

Proper citation

Novus Cat#NB110-40553, RRID:AB_2168319

Product name

Rabbit Polyclonal Calcineurin A Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Calcineurin A Antibody [NB110-40553] - Detection of Human and Mouse PPP3CA by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP3CA antibody NB110-40553 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Calcineurin A Antibody [NB110-40553] - Detection of human and mouse PPP3CA by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP3CA antibody NB110-40553 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: Calcineurin A Antibody [NB110-40553] - Detection of human Calcineurin A (PPP3CA) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Calcineurin A antibody NB110-40553 used for IP at 3 ug per reaction. Calcineurin A was also immunoprecipitated by rabbit anti-Calcineurin A antibody NB110-40552. For blotting immunoprecipitated Calcineurin A, NB110-40553 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Calcineurin A Antibody [NB110-40553] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PPP3CA used at a dilution of 1:1,000 (1ug/ml). Detection: DAB