MAB3235

antibody from R&D Systems

Targeting: NFASC FLJ46866, KIAA0756, NF, NRCAML

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3235

Provider

R&D Systems

Product name

Human/Mouse/Rat Neurofascin Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects rat Neurofascin in direct ELISAs and Western blots.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

NP_446361


Isotype

IgG

Antibody clone number

727030

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

IgG4 anti-neurofascin155 antibodies in chronic inflammatory demyelinating polyradiculoneuropathy: Clinical significance and diagnostic utility of a conventional assay.

Kadoya M, Kaida K, Koike H, Takazaki H, Ogata H, Moriguchi K, Shimizu J, Nagata E, Takizawa S, Chiba A, Yamasaki R, Kira JI, Sobue G, Ikewaki K
Journal of neuroimmunology 2016 Dec 15;301:16-22

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human, Mouse, and Rat Neurofascin by Western Blot. Western blot shows lysates of rat brain tissue, mouse brain tissue, human brain (cortex) tissue, human brain (cerebellum) tissue, and human brain (hippocampus) tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Neurofascin Monoclonal Antibody (Catalog # MAB3235) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Neurofascin at approximately 140 to 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.