Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NBP1-28718 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-28718, RRID:AB_1913934
- Product name
- Rabbit Polyclonal CEP192 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis.
Lee K, Rhee K
The Journal of cell biology 2011 Dec 26;195(7):1093-101
The Journal of cell biology 2011 Dec 26;195(7):1093-101
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: CEP192 Antibody [NBP1-28718] - Detection of Human CEP192 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CEP192 antibody NBP1-28718 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. CEP192 was also immunoprecipitated by rabbit anti-CEP192 antibody BL6868, which recognizes an upstream epitope. For blotting immunoprecipitated CEP192, the ReliaBLOT® Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).