20127-1-AP

antibody from Proteintech Group

Targeting: CYRIB BM-009, CYRI, CYRI-B, FAM49B

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

20127-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#20127-1-AP, RRID:AB_10638431

Product name

FAM49B antibody

Antibody type

Polyclonal

Description

KD/KO validated FAM49B antibody (Cat. #20127-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

FAM49B, restrained by miR-22, relieved hepatic ischemia/reperfusion injury by inhibiting TRAF6/IKK signaling pathway in a Rac1-dependent manner.

Huang Z, Pu J, Luo Y, Fan J, Li K, Peng D, Zong K, Zhou B, Guan X, Zhou F
Molecular immunology 2022 Mar;143:135-146

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Characterization and Functional Study of FAM49B Reveals Its Effect on Cell Proliferation in HEK293T Cells.

Chen Y, Jiang Y, Lao J, Zhou Y, Su L, Huang X
Genes 2022 Feb 21;13(2)

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Fam49/CYRI interacts with Rac1 and locally suppresses protrusions.

Fort L, Batista JM, Thomason PA, Spence HJ, Whitelaw JA, Tweedy L, Greaves J, Martin KJ, Anderson KI, Brown P, Lilla S, Neilson MP, Tafelmeyer P, Zanivan S, Ismail S, Bryant DM, Tomkinson NCO, Chamberlain LH, Mastick GS, Insall RH, Machesky LM
Nature cell biology 2018 Oct;20(10):1159-1171

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse brain tissue were subjected to SDS PAGE followed by western blot with 20127-1-AP(FAM49B antibody) at dilution of 1:500

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using FAM49B antibody 20127-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 20127-1-AP(FAM49B antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 20127-1-AP(FAM49B antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue