Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX22864 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX22864, RRID:AB_384883
- Product name
- CACNA2D1 antibody [20A]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Guinea Pig, Porcine, Rabbit
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Western blot analysis of Cav1.1 in 25 ug of U87-MG and mouse brain lysates. Proteins were transferred to a PVDF membrane and blocked at 4¢XC overnight. The membrane was probed with Cav1.1 antibody [1A] at a dilution of 1:1000 overnight at 4¢XC, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cav1.1 in HeLa cells. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Cav1.1 antibody [1A] at a dilution of 1:100 overnight at 4¢XC, washed with PBS and incubated with a proper secondary antibody. Cav1.1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Cav1.1 in C6 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with Cav1.1 antibody [1A] at a dilution of 1:100 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a proper secondary antibody and re-suspended in PBS for FACS analysis.