PA5-77306
antibody from Invitrogen Antibodies
Targeting: CACNA2D1
CACNA2, CACNL2A, LINC01112, lncRNA-N3, MHS3
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- PA5-77306 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CACNA2D1 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Rat, Rabbit
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.85 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membranes with CACNA2D1 (extracellular) polyclonal antibody (Product # PA5-77306) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of CACNA2D1 was achieved by transfecting SH-SY5Y cells with CACNA2D1 specific siRNAs (Silencer® select Product # s811). Western blot analysis (Fig. a) was performed on membrane enriched extracts from the SH-SY5Y knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with CACNA2D1 (extracellular) Polyclonal Antibody (Product # PA5-77306, 1:200 dilution) and Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 0.25 ug/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to CACNA2D1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CACNA2D1 (extracellular) Rabbit Polyclonal Antibody (Product # PA5-77306) and a 160kDa band corresponding to CACNA2D1 was observed across cell lines tested except Jurkat and K-562 which are reported negative for CACNA2D1. Membrane enriched cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), PC-12 (Lane 2), PC-12 differentiated to neurons (Lane 3), HEK-293 (Lane 4), Jurkat (Lane 5) and K-562 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:200 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).