Antibody data
- Antibody Data
- Antigen structure
- References [29]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- AF1700 - Provider product page
- Provider
- R&D Systems
- Product name
- Human GATA-6 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human GATA-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human GATA-5 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q92908
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation.
yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs.
Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys.
OCT4/POU5F1 is required for NANOG expression in bovine blastocysts.
The miR-590/Acvr2a/Terf1 Axis Regulates Telomere Elongation and Pluripotency of Mouse iPSCs.
Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming.
GATA6 phosphorylation by Erk1/2 propels exit from pluripotency and commitment to primitive endoderm.
ICM conversion to epiblast by FGF/ERK inhibition is limited in time and requires transcription and protein degradation.
Distinct Requirements for FGFR1 and FGFR2 in Primitive Endoderm Development and Exit from Pluripotency.
Lineage Establishment and Progression within the Inner Cell Mass of the Mouse Blastocyst Requires FGFR1 and FGFR2.
Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State.
Abrogation of Gap Junctional Communication in ES Cells Results in a Disruption of Primitive Endoderm Formation in Embryoid Bodies.
The chromatin modifier Satb1 regulates cell fate through Fgf signalling in the early mouse embryo.
p38 (Mapk14/11) occupies a regulatory node governing entry into primitive endoderm differentiation during preimplantation mouse embryo development.
Asynchronous fate decisions by single cells collectively ensure consistent lineage composition in the mouse blastocyst.
Dynamic Heterogeneity of Brachyury in Mouse Epiblast Stem Cells Mediates Distinct Response to Extrinsic Bone Morphogenetic Protein (BMP) Signaling.
Efficient derivation of extraembryonic endoderm stem cell lines from mouse postimplantation embryos.
Epigenetic regulation of histone modifications and Gata6 gene expression induced by maternal diet in mouse embryoid bodies in a model of developmental programming.
GATA family members as inducers for cellular reprogramming to pluripotency.
Inhibition of transforming growth factor β (TGF-β) signaling can substitute for Oct4 protein in reprogramming and maintain pluripotency.
Formation of a polarised primitive endoderm layer in embryoid bodies requires fgfr/erk signalling.
GATA6 levels modulate primitive endoderm cell fate choice and timing in the mouse blastocyst.
Mouse early extra-embryonic lineages activate compensatory endocytosis in response to poor maternal nutrition.
BMP signalling regulates the pre-implantation development of extra-embryonic cell lineages in the mouse embryo.
GATA6 promotes angiogenic function and survival in endothelial cells by suppression of autocrine transforming growth factor beta/activin receptor-like kinase 5 signaling.
A role for PDGF signaling in expansion of the extra-embryonic endoderm lineage of the mouse blastocyst.
A Gata6-Wnt pathway required for epithelial stem cell development and airway regeneration.
New cell lines from mouse epiblast share defining features with human embryonic stem cells.
The human mucin MUC4 is transcriptionally regulated by caudal-related homeobox, hepatocyte nuclear factors, forkhead box A, and GATA endodermal transcription factors in epithelial cancer cells.
Zhang W, Chronis C, Chen X, Zhang H, Spalinskas R, Pardo M, Chen L, Wu G, Zhu Z, Yu Y, Yu L, Choudhary J, Nichols J, Parast MM, Greber B, Sahlén P, Plath K
Cell stem cell 2019 Jan 3;24(1):138-152.e8
Cell stem cell 2019 Jan 3;24(1):138-152.e8
yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs.
Frank S, Ahuja G, Bartsch D, Russ N, Yao W, Kuo JC, Derks JP, Akhade VS, Kargapolova Y, Georgomanolis T, Messling JE, Gramm M, Brant L, Rehimi R, Vargas NE, Kuroczik A, Yang TP, Sahito RGA, Franzen J, Hescheler J, Sachinidis A, Peifer M, Rada-Iglesias A, Kanduri M, Costa IG, Kanduri C, Papantonis A, Kurian L
Cell stem cell 2019 Feb 7;24(2):318-327.e8
Cell stem cell 2019 Feb 7;24(2):318-327.e8
Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys.
Kang Y, Ai Z, Duan K, Si C, Wang Y, Zheng Y, He J, Yin Y, Zhao S, Niu B, Zhu X, Liu L, Xiang L, Zhang L, Niu Y, Ji W, Li T
Cell reports 2018 Nov 27;25(9):2563-2576.e9
Cell reports 2018 Nov 27;25(9):2563-2576.e9
OCT4/POU5F1 is required for NANOG expression in bovine blastocysts.
Simmet K, Zakhartchenko V, Philippou-Massier J, Blum H, Klymiuk N, Wolf E
Proceedings of the National Academy of Sciences of the United States of America 2018 Mar 13;115(11):2770-2775
Proceedings of the National Academy of Sciences of the United States of America 2018 Mar 13;115(11):2770-2775
The miR-590/Acvr2a/Terf1 Axis Regulates Telomere Elongation and Pluripotency of Mouse iPSCs.
Liu Q, Wang G, Lyu Y, Bai M, Jiapaer Z, Jia W, Han T, Weng R, Yang Y, Yu Y, Kang J
Stem cell reports 2018 Jul 10;11(1):88-101
Stem cell reports 2018 Jul 10;11(1):88-101
Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming.
Senft AD, Costello I, King HW, Mould AW, Bikoff EK, Robertson EJ
Cell reports 2018 Aug 21;24(8):1977-1985.e7
Cell reports 2018 Aug 21;24(8):1977-1985.e7
GATA6 phosphorylation by Erk1/2 propels exit from pluripotency and commitment to primitive endoderm.
Meng Y, Moore R, Tao W, Smith ER, Tse JD, Caslini C, Xu XX
Developmental biology 2018 Apr 1;436(1):55-65
Developmental biology 2018 Apr 1;436(1):55-65
ICM conversion to epiblast by FGF/ERK inhibition is limited in time and requires transcription and protein degradation.
Bessonnard S, Coqueran S, Vandormael-Pournin S, Dufour A, Artus J, Cohen-Tannoudji M
Scientific reports 2017 Sep 25;7(1):12285
Scientific reports 2017 Sep 25;7(1):12285
Distinct Requirements for FGFR1 and FGFR2 in Primitive Endoderm Development and Exit from Pluripotency.
Molotkov A, Mazot P, Brewer JR, Cinalli RM, Soriano P
Developmental cell 2017 Jun 5;41(5):511-526.e4
Developmental cell 2017 Jun 5;41(5):511-526.e4
Lineage Establishment and Progression within the Inner Cell Mass of the Mouse Blastocyst Requires FGFR1 and FGFR2.
Kang M, Garg V, Hadjantonakis AK
Developmental cell 2017 Jun 5;41(5):496-510.e5
Developmental cell 2017 Jun 5;41(5):496-510.e5
Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State.
Li X, Liu D, Ma Y, Du X, Jing J, Wang L, Xie B, Sun D, Sun S, Jin X, Zhang X, Zhao T, Guan J, Yi Z, Lai W, Zheng P, Huang Z, Chang Y, Chai Z, Xu J, Deng H
Cell stem cell 2017 Aug 3;21(2):264-273.e7
Cell stem cell 2017 Aug 3;21(2):264-273.e7
Abrogation of Gap Junctional Communication in ES Cells Results in a Disruption of Primitive Endoderm Formation in Embryoid Bodies.
Wörsdörfer P, Bosen F, Gebhardt M, Russ N, Zimmermann K, Komla Kessie D, Sekaran T, Egert A, Ergün S, Schorle H, Pfeifer A, Edenhofer F, Willecke K
Stem cells (Dayton, Ohio) 2017 Apr;35(4):859-871
Stem cells (Dayton, Ohio) 2017 Apr;35(4):859-871
The chromatin modifier Satb1 regulates cell fate through Fgf signalling in the early mouse embryo.
Goolam M, Zernicka-Goetz M
Development (Cambridge, England) 2017 Apr 15;144(8):1450-1461
Development (Cambridge, England) 2017 Apr 15;144(8):1450-1461
p38 (Mapk14/11) occupies a regulatory node governing entry into primitive endoderm differentiation during preimplantation mouse embryo development.
Thamodaran V, Bruce AW
Open biology 2016 Sep;6(9)
Open biology 2016 Sep;6(9)
Asynchronous fate decisions by single cells collectively ensure consistent lineage composition in the mouse blastocyst.
Saiz N, Williams KM, Seshan VE, Hadjantonakis AK
Nature communications 2016 Nov 18;7:13463
Nature communications 2016 Nov 18;7:13463
Dynamic Heterogeneity of Brachyury in Mouse Epiblast Stem Cells Mediates Distinct Response to Extrinsic Bone Morphogenetic Protein (BMP) Signaling.
Song L, Chen J, Peng G, Tang K, Jing N
The Journal of biological chemistry 2016 Jul 15;291(29):15212-25
The Journal of biological chemistry 2016 Jul 15;291(29):15212-25
Efficient derivation of extraembryonic endoderm stem cell lines from mouse postimplantation embryos.
Lin J, Khan M, Zapiec B, Mombaerts P
Scientific reports 2016 Dec 19;6:39457
Scientific reports 2016 Dec 19;6:39457
Epigenetic regulation of histone modifications and Gata6 gene expression induced by maternal diet in mouse embryoid bodies in a model of developmental programming.
Sun C, Denisenko O, Sheth B, Cox A, Lucas ES, Smyth NR, Fleming TP
BMC developmental biology 2015 Jan 21;15:3
BMC developmental biology 2015 Jan 21;15:3
GATA family members as inducers for cellular reprogramming to pluripotency.
Shu J, Zhang K, Zhang M, Yao A, Shao S, Du F, Yang C, Chen W, Wu C, Yang W, Sun Y, Deng H
Cell research 2015 Feb;25(2):169-80
Cell research 2015 Feb;25(2):169-80
Inhibition of transforming growth factor β (TGF-β) signaling can substitute for Oct4 protein in reprogramming and maintain pluripotency.
Tan F, Qian C, Tang K, Abd-Allah SM, Jing N
The Journal of biological chemistry 2015 Feb 13;290(7):4500-11
The Journal of biological chemistry 2015 Feb 13;290(7):4500-11
Formation of a polarised primitive endoderm layer in embryoid bodies requires fgfr/erk signalling.
Doughton G, Wei J, Tapon N, Welham MJ, Chalmers AD
PloS one 2014;9(4):e95434
PloS one 2014;9(4):e95434
GATA6 levels modulate primitive endoderm cell fate choice and timing in the mouse blastocyst.
Schrode N, Saiz N, Di Talia S, Hadjantonakis AK
Developmental cell 2014 May 27;29(4):454-67
Developmental cell 2014 May 27;29(4):454-67
Mouse early extra-embryonic lineages activate compensatory endocytosis in response to poor maternal nutrition.
Sun C, Velazquez MA, Marfy-Smith S, Sheth B, Cox A, Johnston DA, Smyth N, Fleming TP
Development (Cambridge, England) 2014 Mar;141(5):1140-50
Development (Cambridge, England) 2014 Mar;141(5):1140-50
BMP signalling regulates the pre-implantation development of extra-embryonic cell lineages in the mouse embryo.
Graham SJ, Wicher KB, Jedrusik A, Guo G, Herath W, Robson P, Zernicka-Goetz M
Nature communications 2014 Dec 16;5:5667
Nature communications 2014 Dec 16;5:5667
GATA6 promotes angiogenic function and survival in endothelial cells by suppression of autocrine transforming growth factor beta/activin receptor-like kinase 5 signaling.
Froese N, Kattih B, Breitbart A, Grund A, Geffers R, Molkentin JD, Kispert A, Wollert KC, Drexler H, Heineke J
The Journal of biological chemistry 2011 Feb 18;286(7):5680-90
The Journal of biological chemistry 2011 Feb 18;286(7):5680-90
A role for PDGF signaling in expansion of the extra-embryonic endoderm lineage of the mouse blastocyst.
Artus J, Panthier JJ, Hadjantonakis AK
Development (Cambridge, England) 2010 Oct;137(20):3361-72
Development (Cambridge, England) 2010 Oct;137(20):3361-72
A Gata6-Wnt pathway required for epithelial stem cell development and airway regeneration.
Zhang Y, Goss AM, Cohen ED, Kadzik R, Lepore JJ, Muthukumaraswamy K, Yang J, DeMayo FJ, Whitsett JA, Parmacek MS, Morrisey EE
Nature genetics 2008 Jul;40(7):862-70
Nature genetics 2008 Jul;40(7):862-70
New cell lines from mouse epiblast share defining features with human embryonic stem cells.
Tesar PJ, Chenoweth JG, Brook FA, Davies TJ, Evans EP, Mack DL, Gardner RL, McKay RD
Nature 2007 Jul 12;448(7150):196-9
Nature 2007 Jul 12;448(7150):196-9
The human mucin MUC4 is transcriptionally regulated by caudal-related homeobox, hepatocyte nuclear factors, forkhead box A, and GATA endodermal transcription factors in epithelial cancer cells.
Jonckheere N, Vincent A, Perrais M, Ducourouble MP, Male AK, Aubert JP, Pigny P, Carraway KL, Freund JN, Renes IB, Van Seuningen I
The Journal of biological chemistry 2007 Aug 3;282(31):22638-50
The Journal of biological chemistry 2007 Aug 3;282(31):22638-50
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human GATA-6 by Western Blot. Western blot shows cytoplasmic and nuclear extracts from PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GATA-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GATA-6 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- GATA-6 in KATO-III Human Cell Line. GATA-6 was detected in immersion fixed KATO-III human gastric carcinoma cell line using 10 µg/mL Goat Anti-Human GATA-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- GATA-6 in Human Intestine. GATA-6 was detected in immersion fixed paraffin-embedded sections of human intestine using Goat Anti-Human GATA-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of GATA-6-regulated Genes by Chromatin Immunoprecipitation. KATO-III human gastric carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GATA-6/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human GATA-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The mucin4 promoter was detected by standard PCR.