MA5-19153

antibody from Invitrogen Antibodies

Targeting: HSPA4 HS24/P52, HSPH2

ELISA

Antibody data

Submit

Validation data

Product number: MA5-19153 - Provider product page
Provider: Invitrogen Antibodies
Product name: HSPA4 Monoclonal Antibody (3A11)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: Peptide Sequence: MSVVGIDLGF QSCYVAVARA GGIETIANEY SDRCTPACIS FGPKNRSIGA AAKSQVISNA KNTVQGFKRF HGRAFSDPFV EAEKSNLAYD IVQLPTGLTG IKVTYMEEEQ NGPVDGQGDN PGPQAAEQGT DTAVPSDSDK KLPEMDID
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 3A11
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

HSPA4 protein structure - MA5-19153 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of HSPA4 expressed in transfected 293T cell line by anti-HSPA4 monoclonal antibody (Product # MA5-19153), clone 3A11. Lane 1: HSPA4 transfected lysate (94.3 KDa). Lane 2: Non-transfected lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of HSPA4 was achieved by transfecting Hep G2 with HSPA4 specific siRNAs (Silencer® select Product # s6976, s6978). Western blot analysis (Fig. a) was performed using whole cell extracts from the HSPA4 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with HSPA4 Monoclonal Antibody (3A11) (Product # MA5-19153, 1ug/ml dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to HSPA4.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-HSPA4 Mouse Monoclonal Antibody (3A11) (Product # MA5-19153) and a 110kDa band corresponding to HSPA4 was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), HEK-293 (Lane 3), A549 (Lane 4), SK-BR-3 (Lane 5), NIH/3T3 (Lane 6), PC-12 (Lane 7), COS-7 (Lane 8), MDCK (Lane 9), Mouse Testis (Lane 10) and Rat Ovary (Lane 11) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1ug/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).