Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- MA5-24843 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSPA4 Recombinant Rabbit Monoclonal Antibody (3B3)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 3B3
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSPA4 in Lane 1: MCF-7 whole cell lysate, Lane 2: HCT116 whole cell lysate. Samples were incubated with HSPA4 monoclonal antibody (Product # MA5-24843) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSPA4 in Lane 1: MCF-7 whole cell lysate, Lane 2: HCT116 whole cell lysate. Samples were incubated with HSPA4 monoclonal antibody (Product # MA5-24843) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HSPA4 in MCF7 cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with HSPA4 monoclonal antibody (Product # MA5-24843).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HSPA4 in HeLa cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with HSPA4 monoclonal antibody (Product # MA5-24843).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HSPA4 in paraffin-embedded mouse testis tissue. Samples were incubated with HSPA4 monoclonal antibody (Product # MA5-24843) followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HSPA4 in paraffin-embedded mouse prostate tissue. Samples were incubated with HSPA4 monoclonal antibody (Product # MA5-24843) followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HSPA4 in paraffin-embedded mouse brain tissue. Samples were incubated with HSPA4 monoclonal antibody (Product # MA5-24843) followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HSPA4 in paraffin-embedded human breast carcinoma tissue. Samples were incubated with HSPA4 monoclonal antibody (Product # MA5-24843) followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HSPA4 in HeLa cells (blue) and compared with an unlabeled control (cells without incubation with primary antibody; red). Samples were incubated with HSPA4 monoclonal antibody (Product # MA5-24843) at a dilution of 1:50 followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.