MA5-41051

antibody from Invitrogen Antibodies

Targeting: HSPA4 HS24/P52, HSPH2

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-41051

Provider

Invitrogen Antibodies

Product name

HSP70 Recombinant Rabbit Monoclonal Antibody (001)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

1

Vial size

100 µL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of Human HSP70 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with HSP70 Recombinant Rabbit Monoclonal Antibody (001) (Product # MA5-41051, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of HSP70 in HeLa cells. Samples were treated with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, incubated with monoclonal antibody (Product # MA5-41051) with a dilution of 1:60 (37°C 1 hour), followed by Alexa Fluor 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of Human HSP70 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with HSP70 Recombinant Rabbit Monoclonal Antibody (001) (Product # MA5-41051, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HSP70 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate 2 µL with HSP70 Recombinant Rabbit Monoclonal Antibody (001) (Product # MA5-41051) and 15 µL of 50 % Protein G agarose. Primary antibody: HSP70 Recombinant Rabbit Monoclonal Antibody (001), at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 70 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of HSP70 in Lane A: 0.5 mg Hela Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with monoclonal antibody (Product # MA5-41051) with a dilution of 1:200 , followed by Dylight 800-labeled antibody to rabbit IgG (H+L) using a dilution of 1:5,000. Assay was performed under reducing conditions. Predicted band size: 70 kDa, Observed band size: 70 kDa .

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) of HSP70 in cynomolgus macaque testis. Samples were incubated with monoclonal antibody (Product # MA5-41051) with a dilution of 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of HSP70 in human hepatoma with HSP70 Recombinant Rabbit Monoclonal Antibody (001) (Product # MA5-41051, 1:1,000, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) of HSP70 in human hepatoma. Samples were incubated with monoclonal antibody (Product # MA5-41051) with a dilution of 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of HSP70 in cynomolgus macaque testis with HSP70 Recombinant Rabbit Monoclonal Antibody (001) (Product # MA5-41051, 1:1,000, formalin-fixed paraffin embedded sections).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Human HSP70 expression on HeLa cells. The cells were treated according to manufacturer’s manual, stained with HSP70 Recombinant Rabbit Monoclonal Antibody (001) (Product # MA5-41051), then a FITC-conjugated Secondary antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry of HSP70 in HeLa cells. Samples were incubated with monoclonal antibody (Product # MA5-41051) followed by FITC.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Human HSP70 expression on HeLa cells. The cells were treated according to manufacturer’s manual, stained with HSP70 Recombinant Rabbit Monoclonal Antibody (001) (Product # MA5-41051), then a FITC-conjugated Secondary antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of HSP70 in Lane A: 0.5 mg Hela Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with monoclonal antibody (Product # MA5-41051) with a dilution of 1:200 , followed by Dylight 800-labeled antibody to rabbit IgG (H+L) using a dilution of 1:5,000. Assay was performed under reducing conditions. Predicted band size: 70 kDa, Observed band size: 70 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HSP70 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate 2 µL with HSP70 Recombinant Rabbit Monoclonal Antibody (001) (Product # MA5-41051) and 15 µL of 50 % Protein G agarose. Primary antibody: HSP70 Recombinant Rabbit Monoclonal Antibody (001), at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 70 kDa.