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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 648002 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#648002, RRID:AB_2264228
- Product name
- Purified anti-Hsp70
- Antibody type
- Monoclonal
- Antigen
- Recombinant Hsp70 protein
- Description
- Hsp70 is a member of the Hsp family of highly conserved proteins that play a role in a number of important functions. Their synthesis is increased upon environmental stress. They function by acting as molecular chaperones, facilitating protein refolding and preventing protein aggregation. It has been reported that extracellular Hsp70 also exhibits immunomodulatory effects on innate and acquired immunity.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4 °C.
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- NIH/3T3 (lane 1) and Hela (lane 2) cell lysate were resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Hsp70 antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.
- Conjugate
- Unconjugated
