Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
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- Product number
- PA5-30718 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Chromogranin C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: IMR32, U87-MG, mouse brain, rat brain. Predicted reactivity: Mouse (89%), Rat (88%), Pig (88%), Rhesus Monkey (96%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Chromogranin C Polyclonal Antibody (Product # PA5-30718). Sample (30 µg of whole cell lysate). Lane A: IMR32. Lane B: U87-MG. 7.5% SDS PAGE. Chromogranin C Polyclonal Antibody (Product # PA5-30718) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromogranin C Polyclonal Antibody detects SCG2 protein by western blot analysis. A. 50 µg mouse brain lysate/extract.7.5% SDS-PAGE. Chromogranin C Polyclonal Antibody (Product # PA5-30718) dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromogranin C Polyclonal Antibody detects SCG2 protein by western blot analysis. A. 50 µg rat brain lysate/extract.7.5% SDS-PAGE. Chromogranin C Polyclonal Antibody (Product # PA5-30718) dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromogranin C Polyclonal Antibody detects Chromogranin C protein at membrane on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Chromogranin C Polyclonal Antibody (Product # PA5-30718) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromogranin C Polyclonal Antibody detects SCG2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Chromogranin C Polyclonal Antibody (Product # PA5-30718) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromogranin C Polyclonal Antibody detects SCG2 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Chromogranin C Polyclonal Antibody (Product # PA5-30718) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.