PA5-30718

antibody from Invitrogen Antibodies

Targeting: SCG2 CHGC, SgII, SN

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30718

Provider

Invitrogen Antibodies

Product name

Chromogranin C Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: IMR32, U87-MG, mouse brain, rat brain.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Chromogranin C Polyclonal Antibody (Product # PA5-30718). Sample (30 µg of whole cell lysate). Lane A: IMR32. Lane B: U87-MG. 7.5% SDS PAGE. Chromogranin C Polyclonal Antibody (Product # PA5-30718) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromogranin C Polyclonal Antibody detects SCG2 protein by western blot analysis. A. 50 µg mouse brain lysate/extract.7.5% SDS-PAGE. Chromogranin C Polyclonal Antibody (Product # PA5-30718) dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromogranin C Polyclonal Antibody detects SCG2 protein by western blot analysis. A. 50 µg rat brain lysate/extract.7.5% SDS-PAGE. Chromogranin C Polyclonal Antibody (Product # PA5-30718) dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromogranin C Polyclonal Antibody detects Chromogranin C protein at membrane on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Chromogranin C Polyclonal Antibody (Product # PA5-30718) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromogranin C Polyclonal Antibody detects SCG2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Chromogranin C Polyclonal Antibody (Product # PA5-30718) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromogranin C Polyclonal Antibody detects SCG2 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Chromogranin C Polyclonal Antibody (Product # PA5-30718) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.