Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA5-39299 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-NEGR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis was performed on membrane extracts (30 µg lysate) of PC-12 (Lane 1), PC-12 differentiated to neurons upon NGF treatment (100 ng/mL for 2hrs) (Lane 2), U-87 MG (Lane 3), COLO 205 (Lane 4) and tissue extract of Rat Brain (Lane 5). The blot was probed with anti-NEGR1 Polyclonal Antibody (Product # PA5-39299, 1:1000 dilution) and detected by chemiluminescence using Goat anti Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 38 kDa band corresponding to NEGR1 was detected across the cell lines and tissue tested.