Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- BAF3597 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse RGM-B Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse RGM-B in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant mouse (rm) RGM-C is observed and less than 2% cross-reactivity with rmRGM-A is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Biotin
- Antigen sequence
Q7TQ33
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references B7-DC (PD-L2) costimulation of CD4+ T-helper 1 response via RGMb.
Nie X, Chen W, Zhu Y, Huang B, Yu W, Wu Z, Guo S, Zhu Y, Luo L, Wang S, Chen L
Cellular & molecular immunology 2018 Oct;15(10):888-897
Cellular & molecular immunology 2018 Oct;15(10):888-897
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- RGM-B in Neuro-2A Mouse Cell Line. RGM-B was detected in immersion fixed Neuro-2A mouse neuroblastoma cell line using Sheep Anti-Mouse RGM-B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3597) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.