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LearnPA5-47860
antibody from Invitrogen Antibodies
Targeting: B4GAT1
B3GN-T1, B3GNT1, B3GNT6, BETA3GNTI, iGAT, iGNT
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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- Product number
- PA5-47860 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- B3GNT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) B4GalT1 and rhB3GNT 2 is observed. Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human muscle tissue and mouse muscle tissue. PVDF Membrane was probed with 1 µg/mL of human ß-1, 3-N-acetylglucosaminyltransferase 1/B3GNT1 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47860) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for ß-1, 3-N-acetylglucosaminyltransferase 1/B3GNT1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of B3GNT1 in human muscle tissue and mouse muscle tissue. Samples were incubated in B3GNT1 polyclonal antibody (Product # PA5-47860) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for β-1,4-Glucuronyltransferase 1/B4GAT1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions.
