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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB6664 - Provider product page
- Provider
- R&D Systems
- Product name
- Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human beta-1,4-Glucuronyltransferase 1/B4GAT1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B3GNT2, rhB3GNT6, or rhB4GalT1 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O43505- Isotype
- IgG
- Antibody clone number
- 724057
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Shedding of glycan-modifying enzymes by signal peptide peptidase-like 3 (SPPL3) regulates cellular N-glycosylation.
Voss M, Künzel U, Higel F, Kuhn PH, Colombo A, Fukumori A, Haug-Kröper M, Klier B, Grammer G, Seidl A, Schröder B, Obst R, Steiner H, Lichtenthaler SF, Haass C, Fluhrer R
The EMBO journal 2014 Dec 17;33(24):2890-905
The EMBO journal 2014 Dec 17;33(24):2890-905
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human beta-1,4-Glucuronyltransferase 1/B4GAT1 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Monoclonal Antibody (Catalog # MAB6664) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for beta-1,4-Glucuronyltransferase 1/B4GAT1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
