PA5-36701

antibody from Invitrogen Antibodies

Targeting: WNK1 HSAN2, HSN2, PPP1R167, PRKWNK1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36701

Provider

Invitrogen Antibodies

Product name

Phospho-WNK1 (Thr60) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 230 kDa.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-WNK1 (Thr60) in Lane 1: HEK293T whole cell lysate (40 µg), Lane 2: HEK293T treated with EGF (100 ng/mL, 30 minutes) whole cell lysate (40 µg), Lane 3: PC12 whole cell lysate (40 µg), Lane 4: AML-12 whole cell lysate (40 µg). Samples were incubated with Phospho-WNK1 (Thr60) polyclonal antibody (Product # PA5-36701) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-WNK1 pThr60 using Phospho-WNK1 pThr60 polyclonal antibody (Product # PA5-36701) at a dilution of 1:500. Lane 1: Hela cell lysate treated with EGF (0.1 ng/mL, 30 min), Lane 2: Raw264.7 cell lysate treated with EGF (0.1 ng/mL, 30 min), Lane 3: H9C2 cell lysate treated with EGF (0.1 ng/mL, 30 min).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Phospho-WNK1 pThr60 in paraffin-embedded human breast carcinoma using Phospho-WNK1 pThr60 polyclonal antibody (Product # PA5-36701) at a dilution of 1:100.