Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-36701 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-WNK1 (Thr60) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 230 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-WNK1 (Thr60) in Lane 1: HEK293T whole cell lysate (40 µg), Lane 2: HEK293T treated with EGF (100 ng/mL, 30 minutes) whole cell lysate (40 µg), Lane 3: PC12 whole cell lysate (40 µg), Lane 4: AML-12 whole cell lysate (40 µg). Samples were incubated with Phospho-WNK1 (Thr60) polyclonal antibody (Product # PA5-36701) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-WNK1 pThr60 using Phospho-WNK1 pThr60 polyclonal antibody (Product # PA5-36701) at a dilution of 1:500. Lane 1: Hela cell lysate treated with EGF (0.1 ng/mL, 30 min), Lane 2: Raw264.7 cell lysate treated with EGF (0.1 ng/mL, 30 min), Lane 3: H9C2 cell lysate treated with EGF (0.1 ng/mL, 30 min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-WNK1 pThr60 in paraffin-embedded human breast carcinoma using Phospho-WNK1 pThr60 polyclonal antibody (Product # PA5-36701) at a dilution of 1:100.
