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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB4720-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal WNK1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human WNK1 when phosphorylated at T60 in Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human Phospho-WNK1 (T60) by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line untreated (-) or treated (+) with 1 μg/mL insulin for 5 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-WNK1 (T60) Monoclonal Antibody (Catalog # MAB4720) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-WNK1 (T60) at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
