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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- MA5-50421 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WNK1 Recombinant Rabbit Monoclonal Antibody (HL2184)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- HL2184
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of WNK1 using WNK1 Recombinant Monoclonal Antibody (Product # MA5-50421) in HeLa cells at a dilution of 1:500. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: WNK1 stained by WNK1 antibody (MA5-50421) diluted at 1:500. Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of WNK1 using WNK1 Recombinant Monoclonal Antibody (Product # MA5-50421) in DIV9 rat E18 primary cortical neuron cells at a dilution of 1:500. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: WNK1 stained by WNK1 antibody (MA5-50421) diluted at 1:250. Red: Tau, a axon marker, stained by Tau antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of WNK1 using WNK1 Recombinant Monoclonal Antibody (Product # MA5-50421) in Paraffin embedded mouse kidney tissue at a dilution of 1:50. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of WNK1 using WNK1 Recombinant Monoclonal Antibody (Product # MA5-50421) in Paraffin embedded rat cerebellum tissue at a dilution of 1:50. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.
