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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21860 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ORP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2, mouse brain.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ORP-1 using 50 µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an ORP-1 polyclonal antibody (Product # PA5-21860) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ORP1 Polyclonal Antibody (Product # PA5-21860). Sample (30 µg of whole cell lysate). Lane A: H1299. 10% SDS PAGE. ORP1 Polyclonal Antibody (Product # PA5-21860) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ORP1 Polyclonal Antibody (Product # PA5-21860). Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ORP1 Polyclonal Antibody (Product # PA5-21860) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ORP-1 in methanol-fixed HeLa cells using an ORP-1 polyclonal antibody (Product # PA5-21860) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using ORP1 (Product # PA5-21860) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ORP1 (Product # PA5-21860) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
