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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NB100-1331 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1331, RRID:AB_2251874
- Product name
- Goat Polyclonal ORP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise both reported human isoforms of this protein.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Intracellular cholesterol-binding proteins enhance HDL-mediated cholesterol uptake in cultured primary mouse hepatocytes.
Adenovirus RIDalpha regulates endosome maturation by mimicking GTP-Rab7.
The OSBP-related protein family in humans.
Storey SM, McIntosh AL, Huang H, Landrock KK, Martin GG, Landrock D, Payne HR, Atshaves BP, Kier AB, Schroeder F
American journal of physiology. Gastrointestinal and liver physiology 2012 Apr 15;302(8):G824-39
American journal of physiology. Gastrointestinal and liver physiology 2012 Apr 15;302(8):G824-39
Adenovirus RIDalpha regulates endosome maturation by mimicking GTP-Rab7.
Shah AH, Cianciola NL, Mills JL, Sönnichsen FD, Carlin C
The Journal of cell biology 2007 Dec 3;179(5):965-80
The Journal of cell biology 2007 Dec 3;179(5):965-80
The OSBP-related protein family in humans.
Lehto M, Laitinen S, Chinetti G, Johansson M, Ehnholm C, Staels B, Ikonen E, Olkkonen VM
Journal of lipid research 2001 Aug;42(8):1203-13
Journal of lipid research 2001 Aug;42(8):1203-13
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ORP1 Antibody [NB100-1331] - Analysis of ORP1 in Human Muscle lysate using NB100-1331 at 0.5 ug/ ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ORP1 Antibody [NB100-1331] - (0.5ug/ml) of Human Muscle lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ORP1 Antibody [NB100-1331] - (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ORP1 Antibody [NB100-1331] - (5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
