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LearnMA5-27717
antibody from Invitrogen Antibodies
Targeting: CACNA1C
CACH2, CACN2, CACNL1A1, Cav1.2, CCHL1A1, LQT8, TS
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- MA5-27717 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CaV1.2 Monoclonal Antibody (S57)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- 1 µg/mL of MA5-27717 was sufficient for detection of Cav1.2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 240kDa (varies with cell background due to glycosylation).
- Reactivity
- Human, Mouse, Rat, Hamster, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- S57
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cav1.2 in Hamster T-CHO cell lysate. The sample was incubated with Cav1.2 monoclonal antibody (Product # MA5-27717) using a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cav1.2 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with Cav1.2 monoclonal antibody (Product # MA5-27717) using a dilution of 1:200 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200 (1 hour at RT). Images are shown as follows: (A) Phalloidin Texas Red F-Actin stain, DAPI (blue) nuclear stain, (B) Anti-Cav1,2 Antibody, (C) Merged image (A) DAPI (blue) nuclear stain, (B) Phalloidin Texas Red F-Actin stain, (C) Cav1,2 Antibody, (D) Merged image. Magnification: 60x.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Cav1.2 in human hippocampus. Sample was fixed using 10% formalin, incubated with Cav1.2 monoclonal antibody (Product # MA5-27717) using a dilution of 1:100 (1 hour at RT), and followed by FITC Goat Anti-Mouse (green) (1 hour at RT) at a dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Cav1.2 in mouse brain tissue. Sample was fixed with formalin, incubated with Cav1.2 monoclonal antibody (Product # MA5-27717) using a dilution of 1:10,000 (12 hours at 4°C), and followed by Biotin Goat Anti-Mouse and Mayer Hematoxylin (purple/blue) for 1 hour at RT at a dilution of 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Cav1.2 in INS-1E cells. Sample was incubated with Cav1.2 monoclonal antibody (Product # MA5-27717) using a dilution of 1:200.
