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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- AF3349a - Provider product page
- Provider
- Abgent
- Product name
- GM2A (aa 164-175) Antibody (internal region)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide-affinity purified goat antibody
- Reactivity
- Mouse, Bovine, Canine, Rabbit
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 0.1mg
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -1050°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human GM2A with DYKDDDDK tag probed with AF3349a (1.0ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/30000) in lane C. Mock-transfected HEK293 probed with AF3349a (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "AF3349a (0.3 µg/ml) staining of Rat Kidney lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. "
