Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- ABIN615967 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-GM2 Ganglioside Activator (GM2A) (Internal Region), (AA 164-175) antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide with sequence (aa 164-175) from the internal region of the protein sequence according to NP_000396.2. Genename: GM2A
- Description
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
- Reactivity
- Human, Mouse, Rat, Rabbit
- Host
- Goat
- Antigen sequence
C-TTGNYRIESVLS
- Epitope
- Internal Region,AA 164-175
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Staining of Rat Kidney lysate using GM2A Antibody Cat.-No AP23748PU-N at 0.3 μg/ml (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- HEK293 lysate (10 μg protein in RIPA buffer) overexpressing Human GM2A with DYKDDDDK tag probed with AP23748PU-N (1.0 μg/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/30000) in Lane C. Mock-transfected HEK293 probed with AP23748PU-N (1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.