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- References [3]
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- Western blot [2]
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- Product number
- HPA035010 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA035010, RRID:AB_10669853
- Product name
- Anti-ATP5I
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human ATP5I, Gene description: ATP synthase, H+ transporting, mitochondrial Fo complex, subunit E, Validated applications: ICC, IHC, WB, Uniprot ID: P56385, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.4 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Sirtuin 3 Deficiency Aggravates Kidney Disease in Response to High-Fat Diet through Lipotoxicity-Induced Mitochondrial Damage
Shiga Toxin 2 Triggers C3a-Dependent Glomerular and Tubular Injury through Mitochondrial Dysfunction in Hemolytic Uremic Syndrome
C3a receptor blockade protects podocytes from injury in diabetic nephropathy
Locatelli M, Macconi D, Corna D, Cerullo D, Rottoli D, Remuzzi G, Benigni A, Zoja C
International Journal of Molecular Sciences 2022;23(15):8345
International Journal of Molecular Sciences 2022;23(15):8345
Shiga Toxin 2 Triggers C3a-Dependent Glomerular and Tubular Injury through Mitochondrial Dysfunction in Hemolytic Uremic Syndrome
Buelli S, Locatelli M, Carminati C, Corna D, Cerullo D, Imberti B, Perico L, Brigotti M, Abbate M, Zoja C, Benigni A, Remuzzi G, Morigi M
Cells 2022;11(11):1755
Cells 2022;11(11):1755
C3a receptor blockade protects podocytes from injury in diabetic nephropathy
Morigi M, Perico L, Corna D, Locatelli M, Cassis P, Carminati C, Bolognini S, Zoja C, Remuzzi G, Benigni A, Buelli S
JCI Insight 2020;5(5)
JCI Insight 2020;5(5)
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Enhanced validation
Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting ATP5I or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting ATP5I Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:328
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot
Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1, using Anti-ATP5I antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
