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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-36578 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP5I Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 8 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATP5I using ATP5I polyclonal antibody (Product # PA5-36578) at a dilution of 1:500. Lane 1: Hela cell lysate, Lane 2: Mouse heart tissue lysate, Lane 3: Rat heart tissue lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATP5I in Lane 1: AML-12 whole cell lysate (40 µg), Lane 2: C6 whole cell lysate (40 µg), Lane 3: HepG2 whole cell lysate (40 µg), Lane 4: PC3 whole cell lysate (40 µg), Lane 5: HEK293T whole cell lysate (40 µg). Samples were incubated with ATP5I polyclonal antibody (Product # PA5-36578) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of ATP5I in HEK293T cell lysate (sepharose bead conjugate), (lane 2 and lane 3) and Nonspecific IgG Control (sepharose bead conjugate, lane 4). Lane 1 is 30% input. Samples were incubated with ATP5I polyclonal antibody (Product # PA5-36578) Results legends show supernatant (upward arrow) and deposition (downward arrow).
