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- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- BS4694 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- hnRNP K (phospho S284) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human hnRNP K around the phosphorylation site of Serine 284.
- Description
- The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K, a 65 kDa protein, recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of p-hnRNP K (S284) pAb at 1:500 dilutionLane1:MCF-7 cell lysate treated with EGF(0.1ng/ML,30mins)Lane2:Raw264.7 cell lysate treated with EGF(0.1ng/ML,30mins)Lane3:H9C2 cell lysate treated with EGF(0.1ng/ML,30mins)
