Antibody data
- Antibody Data
- Antigen structure
- References [39]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 10736-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#10736-1-AP, RRID:AB_2214635
- Product name
- VCP antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated VCP antibody (Cat. #10736-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references New mode of action of curcumin on prostate cancer cells: Modulation of endoplasmic reticulum-associated degradation mechanism and estrogenic signaling.
Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders.
UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation.
hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility.
SEL1L-HRD1 interaction is required to form a functional HRD1 ERAD complex.
Circadian Oscillation Pattern of Endoplasmic Reticulum Quality Control (ERQC) Components in Human Embryonic Kidney HEK293 Cells.
Estrogens drive the endoplasmic reticulum-associated degradation and promote proto-oncogene c-Myc expression in prostate cancer cells by androgen receptor/estrogen receptor signaling.
Triiodothyronine positively regulates endoplasmic reticulum-associated degradation (ERAD) and promotes androgenic signaling in androgen-dependent prostate cancer cells.
G3BP1 coordinates lysophagy activity to protect against compression-induced cell ferroptosis during intervertebral disc degeneration.
1,25(OH)(2) D(3) induced vitamin D receptor signaling negatively regulates endoplasmic reticulum-associated degradation (ERAD) and androgen receptor signaling in human prostate cancer cells.
GLI1, a novel target of the ER stress regulator p97/VCP, promotes ATF6f-mediated activation of XBP1.
Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats.
CircHIPK3 negatively regulates autophagy by blocking VCP binding to the Beclin 1 complex in bladder cancer.
Dexamethasone-stimulated glucocorticoid receptor signaling positively regulates the endoplasmic reticulum-associated degradation (ERAD) mechanism in hepatocellular carcinoma cells.
Progesterone regulates the endoplasmic reticulum-associated degradation and Unfolded Protein Response axis by mimicking the androgenic stimulation in prostate cancer cells.
The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition.
Inhibition of VCP modulates NF-κB signaling pathway to suppress multiple myeloma cell proliferation and osteoclast differentiation.
Valosin-containing protein (VCP/p97) is responsible for the endocytotic trafficking of classical swine fever virus.
UBXD8 mediates mitochondria-associated degradation to restrain apoptosis and mitophagy.
An eIF3d-dependent switch regulates HCMV replication by remodeling the infected cell translation landscape to mimic chronic ER stress.
Targeting non-canonical pathways as a strategy to modulate the sodium iodide symporter.
The Mechano-Ubiquitinome of Articular Cartilage: Differential Ubiquitination and Activation of a Group of ER-Associated DUBs and ER Stress Regulators.
MEST promotes lung cancer invasion and metastasis by interacting with VCP to activate NF-κB signaling.
SAA1 is transcriptionally activated by STAT3 and accelerates renal interstitial fibrosis by inducing endoplasmic reticulum stress.
p97/VCP inhibition causes excessive MRE11-dependent DNA end resection promoting cell killing after ionizing radiation.
Analysis of Zika virus capsid-Aedes aegypti mosquito interactome reveals pro-viral host factors critical for establishing infection.
Hyaluronic acid/polyethyleneimine nanoparticles loaded with copper ion and disulfiram for esophageal cancer.
SPRTN protease-cleaved MRE11 decreases DNA repair and radiosensitises cancer cells.
The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability.
Berberine alleviates liver fibrosis through inducing ferrous redox to activate ROS-mediated hepatic stellate cells ferroptosis.
VPS13D interacts with VCP/p97 and negatively regulates endoplasmic reticulum-mitochondria interactions.
Vitamin D Attenuates Alzheimer-like Pathology Induced by Okadaic Acid.
The p97-UBXN1 complex regulates aggresome formation.
Active Protein Neddylation or Ubiquitylation Is Dispensable for Stress Granule Dynamics.
The VCP-UBXN1 Complex Mediates Triage of Ubiquitylated Cytosolic Proteins Bound to the BAG6 Complex.
AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.
Acupuncture regulates the unfolded protein response and inhibits apoptosis in a rat model of heroin relapse.
Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.
Integrative quantitative proteomics unveils proteostasis imbalance in human hepatocellular carcinoma developed on nonfibrotic livers.
Erzurumlu Y, Dogan HK, Catakli D
Journal of biochemical and molecular toxicology 2024 Jan;38(1):e23636
Journal of biochemical and molecular toxicology 2024 Jan;38(1):e23636
Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders.
Wang HH, Lin LL, Li ZJ, Wei X, Askander O, Cappuccio G, Hashem MO, Hubert L, Munnich A, Alqahtani M, Pang Q, Burmeister M, Lu Y, Poirier K, Besmond C, Sun S, Brunetti-Pierri N, Alkuraya FS, Qi L
The Journal of clinical investigation 2024 Jan 16;134(2)
The Journal of clinical investigation 2024 Jan 16;134(2)
UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation.
Ahlstedt BA, Ganji R, Mukkavalli S, Paulo JA, Gygi SP, Raman M
EMBO reports 2024 Feb;25(2):672-703
EMBO reports 2024 Feb;25(2):672-703
hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility.
Wang X, Fan X, Zhang J, Wang F, Chen J, Wen Y, Wang L, Li T, Li H, Gu H, Zhang Y, Yuan S
Cell reports 2024 Feb 27;43(2):113769
Cell reports 2024 Feb 27;43(2):113769
SEL1L-HRD1 interaction is required to form a functional HRD1 ERAD complex.
Lin LL, Wang HH, Pederson B, Wei X, Torres M, Lu Y, Li ZJ, Liu X, Mao H, Wang H, Zhou LE, Zhao Z, Sun S, Qi L
Nature communications 2024 Feb 16;15(1):1440
Nature communications 2024 Feb 16;15(1):1440
Circadian Oscillation Pattern of Endoplasmic Reticulum Quality Control (ERQC) Components in Human Embryonic Kidney HEK293 Cells.
Erzurumlu Y, Catakli D, Dogan HK
Journal of circadian rhythms 2023;21:1
Journal of circadian rhythms 2023;21:1
Estrogens drive the endoplasmic reticulum-associated degradation and promote proto-oncogene c-Myc expression in prostate cancer cells by androgen receptor/estrogen receptor signaling.
Erzurumlu Y, Dogan HK, Catakli D, Aydogdu E, Muhammed MT
Journal of cell communication and signaling 2023 Sep;17(3):793-811
Journal of cell communication and signaling 2023 Sep;17(3):793-811
Triiodothyronine positively regulates endoplasmic reticulum-associated degradation (ERAD) and promotes androgenic signaling in androgen-dependent prostate cancer cells.
Erzurumlu Y, Muhammed MT
Cellular signalling 2023 Sep;109:110745
Cellular signalling 2023 Sep;109:110745
G3BP1 coordinates lysophagy activity to protect against compression-induced cell ferroptosis during intervertebral disc degeneration.
Li S, Liao Z, Yin H, Liu O, Hua W, Wu X, Zhang Y, Gao Y, Yang C
Cell proliferation 2023 Mar;56(3):e13368
Cell proliferation 2023 Mar;56(3):e13368
1,25(OH)(2) D(3) induced vitamin D receptor signaling negatively regulates endoplasmic reticulum-associated degradation (ERAD) and androgen receptor signaling in human prostate cancer cells.
Erzurumlu Y, Aydogdu E, Dogan HK, Catakli D, Muhammed MT, Buyuksandic B
Cellular signalling 2023 Mar;103:110577
Cellular signalling 2023 Mar;103:110577
GLI1, a novel target of the ER stress regulator p97/VCP, promotes ATF6f-mediated activation of XBP1.
Almada LL, Barroso K, Sen S, Toruner M, Sigafoos AN, Raja Arul GL, Pease DR, Vera RE, Olson RLO, Auner HW, Pedeux R, Iovanna JL, Chevet E, Fernandez-Zapico ME
Biochimica et biophysica acta. Gene regulatory mechanisms 2023 Jun;1866(2):194924
Biochimica et biophysica acta. Gene regulatory mechanisms 2023 Jun;1866(2):194924
Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats.
Tseng YJ, Malik I, Deng X, Krans A, Jansen-West K, Tank EMH, Gomez NB, Sher R, Petrucelli L, Barmada SJ, Todd PK
bioRxiv : the preprint server for biology 2023 Jun 7;
bioRxiv : the preprint server for biology 2023 Jun 7;
CircHIPK3 negatively regulates autophagy by blocking VCP binding to the Beclin 1 complex in bladder cancer.
Wang C, Liu T, Wang J, Cheng C, Zhang Z, Zhang J, Huang H, Li Y
Discover oncology 2023 Jun 3;14(1):86
Discover oncology 2023 Jun 3;14(1):86
Dexamethasone-stimulated glucocorticoid receptor signaling positively regulates the endoplasmic reticulum-associated degradation (ERAD) mechanism in hepatocellular carcinoma cells.
Erzurumlu Y, Dogan HK, Cataklı D
Steroids 2023 Jul;195:109238
Steroids 2023 Jul;195:109238
Progesterone regulates the endoplasmic reticulum-associated degradation and Unfolded Protein Response axis by mimicking the androgenic stimulation in prostate cancer cells.
Erzurumlu Y, Dogan HK, Catakli D
Molecular biology reports 2023 Feb;50(2):1253-1265
Molecular biology reports 2023 Feb;50(2):1253-1265
The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition.
Ganji R, Paulo JA, Xi Y, Kline I, Zhu J, Clemen CS, Weihl CC, Purdy JG, Gygi SP, Raman M
Nature communications 2023 Feb 6;14(1):638
Nature communications 2023 Feb 6;14(1):638
Inhibition of VCP modulates NF-κB signaling pathway to suppress multiple myeloma cell proliferation and osteoclast differentiation.
Wei R, Cao Y, Wu H, Liu X, Jiang M, Luo X, Deng Z, Wang Z, Ke M, Zhu Y, Chen S, Gu C, Yang Y
Aging 2023 Aug 21;15(16):8220-8236
Aging 2023 Aug 21;15(16):8220-8236
Valosin-containing protein (VCP/p97) is responsible for the endocytotic trafficking of classical swine fever virus.
Sun RC, Hu JH, Li XH, Liu CC, Liu YY, Chen J, Yang YC, Zhou B
Veterinary microbiology 2022 Sep;272:109511
Veterinary microbiology 2022 Sep;272:109511
UBXD8 mediates mitochondria-associated degradation to restrain apoptosis and mitophagy.
Zheng J, Cao Y, Yang J, Jiang H
EMBO reports 2022 Oct 6;23(10):e54859
EMBO reports 2022 Oct 6;23(10):e54859
An eIF3d-dependent switch regulates HCMV replication by remodeling the infected cell translation landscape to mimic chronic ER stress.
Thompson L, Depledge DP, Burgess HM, Mohr I
Cell reports 2022 May 3;39(5):110767
Cell reports 2022 May 3;39(5):110767
Targeting non-canonical pathways as a strategy to modulate the sodium iodide symporter.
Read ML, Brookes K, Thornton CEM, Fletcher A, Nieto HR, Alshahrani M, Khan R, Borges de Souza P, Zha L, Webster JRM, Alderwick LJ, Campbell MJ, Boelaert K, Smith VE, McCabe CJ
Cell chemical biology 2022 Mar 17;29(3):502-516.e7
Cell chemical biology 2022 Mar 17;29(3):502-516.e7
The Mechano-Ubiquitinome of Articular Cartilage: Differential Ubiquitination and Activation of a Group of ER-Associated DUBs and ER Stress Regulators.
Kaokhum N, Pinto-Fernández A, Wilkinson M, Kessler BM, Ismail HM
Molecular & cellular proteomics : MCP 2022 Dec;21(12):100419
Molecular & cellular proteomics : MCP 2022 Dec;21(12):100419
MEST promotes lung cancer invasion and metastasis by interacting with VCP to activate NF-κB signaling.
Wang Y, Zhang J, Li YJ, Yu NN, Liu WT, Liang JZ, Xu WW, Sun ZH, Li B, He QY
Journal of experimental & clinical cancer research : CR 2021 Sep 24;40(1):301
Journal of experimental & clinical cancer research : CR 2021 Sep 24;40(1):301
SAA1 is transcriptionally activated by STAT3 and accelerates renal interstitial fibrosis by inducing endoplasmic reticulum stress.
Zhang F, Zhou X, Zou H, Liu L, Li X, Ruan Y, Xie Y, Shi M, Xiao Y, Wang Y, Zhou Y, Wu Y, Guo B
Experimental cell research 2021 Nov 1;408(1):112856
Experimental cell research 2021 Nov 1;408(1):112856
p97/VCP inhibition causes excessive MRE11-dependent DNA end resection promoting cell killing after ionizing radiation.
Kilgas S, Singh AN, Paillas S, Then CK, Torrecilla I, Nicholson J, Browning L, Vendrell I, Konietzny R, Kessler BM, Kiltie AE, Ramadan K
Cell reports 2021 May 25;35(8):109153
Cell reports 2021 May 25;35(8):109153
Analysis of Zika virus capsid-Aedes aegypti mosquito interactome reveals pro-viral host factors critical for establishing infection.
Gestuveo RJ, Royle J, Donald CL, Lamont DJ, Hutchinson EC, Merits A, Kohl A, Varjak M
Nature communications 2021 May 13;12(1):2766
Nature communications 2021 May 13;12(1):2766
Hyaluronic acid/polyethyleneimine nanoparticles loaded with copper ion and disulfiram for esophageal cancer.
Xu R, Zhang K, Liang J, Gao F, Li J, Guan F
Carbohydrate polymers 2021 Jun 1;261:117846
Carbohydrate polymers 2021 Jun 1;261:117846
SPRTN protease-cleaved MRE11 decreases DNA repair and radiosensitises cancer cells.
Na J, Newman JA, Then CK, Syed J, Vendrell I, Torrecilla I, Ellermann S, Ramadan K, Fischer R, Kiltie AE
Cell death & disease 2021 Feb 8;12(2):165
Cell death & disease 2021 Feb 8;12(2):165
The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability.
Ruggiano A, Vaz B, Kilgas S, Popović M, Rodriguez-Berriguete G, Singh AN, Higgins GS, Kiltie AE, Ramadan K
Cell reports 2021 Dec 7;37(10):110080
Cell reports 2021 Dec 7;37(10):110080
Berberine alleviates liver fibrosis through inducing ferrous redox to activate ROS-mediated hepatic stellate cells ferroptosis.
Yi J, Wu S, Tan S, Qin Y, Wang X, Jiang J, Liu H, Wu B
Cell death discovery 2021 Dec 4;7(1):374
Cell death discovery 2021 Dec 4;7(1):374
VPS13D interacts with VCP/p97 and negatively regulates endoplasmic reticulum-mitochondria interactions.
Du Y, Wang J, Xiong J, Fang N, Ji WK
Molecular biology of the cell 2021 Aug 1;32(16):1474-1486
Molecular biology of the cell 2021 Aug 1;32(16):1474-1486
Vitamin D Attenuates Alzheimer-like Pathology Induced by Okadaic Acid.
Pan Y, Zhang Y, Liu N, Lu W, Yang J, Li Y, Liu Z, Wei Y, Lou Y, Kong J
ACS chemical neuroscience 2021 Apr 21;12(8):1343-1350
ACS chemical neuroscience 2021 Apr 21;12(8):1343-1350
The p97-UBXN1 complex regulates aggresome formation.
Mukkavalli S, Klickstein JA, Ortiz B, Juo P, Raman M
Journal of cell science 2021 Apr 1;134(7)
Journal of cell science 2021 Apr 1;134(7)
Active Protein Neddylation or Ubiquitylation Is Dispensable for Stress Granule Dynamics.
Markmiller S, Fulzele A, Higgins R, Leonard M, Yeo GW, Bennett EJ
Cell reports 2019 Apr 30;27(5):1356-1363.e3
Cell reports 2019 Apr 30;27(5):1356-1363.e3
The VCP-UBXN1 Complex Mediates Triage of Ubiquitylated Cytosolic Proteins Bound to the BAG6 Complex.
Ganji R, Mukkavalli S, Somanji F, Raman M
Molecular and cellular biology 2018 Jul 1;38(13)
Molecular and cellular biology 2018 Jul 1;38(13)
AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.
Luo H, Jiang M, Lian G, Liu Q, Shi M, Li TY, Song L, Ye J, He Y, Yao L, Zhang C, Lin ZZ, Zhang CS, Zhao TJ, Jia WP, Li P, Lin SY, Lin SC
Cell metabolism 2018 Apr 3;27(4):843-853.e6
Cell metabolism 2018 Apr 3;27(4):843-853.e6
Acupuncture regulates the unfolded protein response and inhibits apoptosis in a rat model of heroin relapse.
Zhang Y, Cai XH, Zhang RJ, Hou XR, Song XG, Wu SB, Yu S, Cao JP
Acupuncture in medicine : journal of the British Medical Acupuncture Society 2016 Dec;34(6):441-448
Acupuncture in medicine : journal of the British Medical Acupuncture Society 2016 Dec;34(6):441-448
Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.
Tao Z, Wang H, Xia Q, Li K, Li K, Jiang X, Xu G, Wang G, Ying Z
Human molecular genetics 2015 May 1;24(9):2426-41
Human molecular genetics 2015 May 1;24(9):2426-41
Integrative quantitative proteomics unveils proteostasis imbalance in human hepatocellular carcinoma developed on nonfibrotic livers.
Negroni L, Taouji S, Arma D, Pallares-Lupon N, Leong K, Beausang LA, Latterich M, Bossé R, Balabaud C, Schmitter JM, Bioulac-Sage P, Zucman-Rossi J, Rosenbaum J, Chevet E
Molecular & cellular proteomics : MCP 2014 Dec;13(12):3473-83
Molecular & cellular proteomics : MCP 2014 Dec;13(12):3473-83
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10736-1-AP(VCP antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-VCP (IP:10736-1-AP, 4ug; Detection:10736-1-AP 1:1000) with HeLa cells lysate 1505ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 10736-1-AP(VCP antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The VCP antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human VCP. This antibody recognizes human,mouse,rat antigen. The VCP antibody has been validated for the following applications: ELISA, WB, IP, IHC analysis.