10736-1-AP

antibody from Proteintech Group

Targeting: VCP CDC48, IBMPFD, p97, TERA

Provider product page for 10736-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [68]
Comments [0]
Validations
Western blot [1]
Immunoprecipitation [1]
Immunohistochemistry [2]

Submit

Product number: 10736-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10736-1-AP, RRID:AB_2214635
Product name: VCP antibody
Antibody type: Polyclonal
Description: KD/KO validated VCP antibody (Cat. #10736-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

VCP protein structure - 10736-1-AP shown in red.

Submitted references STUB1-VCP/p97 complex regulates mitophagy via fine-tuning of PINK1 levels.

Lin JY, Huang ZB, Zhang SQ, Wu Y, Cheng LJ, Gao X, Lautrup S, Cao SQ, Pan J, Rong D, Ai R, Weng Siong Tan H, Wang L, Wang H, Shen HM, Fang EF, Lu G
Cell reports 2026 Mar 27;45(4):117183

Structure-function relationship of alpha-synuclein fibrillar polymorphs derived from distinct synucleinopathies.

Serdiuk T, Redeker V, Savistchenko J, Neupane S, Haenseler W, Fleischmann Y, Reber V, Keller S, Tiberi C, Bachmann-Gagescu R, Gstaiger M, Braun T, Riek R, Gentleman S, Aguzzi A, de Souza N, Melki R, Picotti P
Molecular systems biology 2026 Mar 11;

Targeting VCP with V8 suppresses glioblastoma development via formation of aggregates and disruption of mitophagy flux.

Cao X, Li Y, Guo B, Liu Y, Wang B, Wang H, Lu J, Wei L, Gao Y, Guo Y, Wu T
Cellular oncology (Dordrecht, Netherlands) 2026 Jan 8;49(1):21

ATI-1 mediated disruption of the VCP-UFL1-Beclin1 axis thwarts autophagy initiation to trigger metabolic catastrophe in autophagy-addicted cancers.

Liu T, Zhao M, Gao J, Liu J, Zhang J
Bioorganic chemistry 2026 Aug 5;177:109923

Locus-specific proteomics identifies novel regulators of Epstein-Barr virus lytic reactivation.

Greaves DE, Gabaev I, Williamson JC, Bloor S, Martinez Cuesta S, Matheson NJ, Frappier L, Déjardin J, Lehner PJ
Journal of virology 2026 Apr 21;100(4):e0140825

Pathogenic mechanisms of amyotrophic lateral sclerosis-linked VAPB P56S mutation in the degeneration of corticospinal motor neurons.

Yang X, Zheng J, Wang X, Cai H, Yu J
Ageing and neurodegenerative diseases 2025;5(3)

Targeting p97/Valosin-Containing Protein Promotes Hepatic Stellate Cell Senescence and Mitigates Liver Fibrosis.

Yang Y, Zhang Y, Wang L, He G, Yang X, Qing J
DNA and cell biology 2025;44(3):132-143

A guide to selecting high-performing antibodies for VCP (UniProt ID: P55072) for use in western blot, immunoprecipitation, and immunofluorescence.

Moleón VR, Fotouhi M, Ryan J, Worrall D, Ayoubi R, Francis V, McPherson PS, Laflamme C
F1000Research 2025;14:891

CRISPR screens identify the ATPase VCP as a druggable therapeutic vulnerability in cholangiocarcinoma.

Yang W, Wang S, Ji S, Wang J, Lian S, Li Z, Jansen RA, Wu W, Niu K, Sun Z, Jia Q, Zheng J, Zhu H, Deng X, Wang L, Fan Z, Shi Y, Lieftink C, Guan M, Beijersbergen RL, Qin W, Gao Q, Bernards R, Jin H
Proceedings of the National Academy of Sciences of the United States of America 2025 Sep 30;122(39):e2519568122

RHBDD1 induces cell cycle arrest in TP53-mutant NSCLC cells by promoting endoplasmic reticulum-associated degradation of p53 and DNA-PKcs.

Chen Y, Wang Y, Shen S, Zhang L, Liu J, Yan X, Peng H, Xu Z
European journal of medical research 2025 Sep 26;30(1):859

Response of UBR-box E3 ubiquitin ligases and protein quality control pathways to perturbations in protein synthesis and skeletal muscle size.

Baehr LM, de Sousa LGO, Goodman CA, Sharples AP, Waddell DS, Bodine SC, Hughes DC
bioRxiv : the preprint server for biology 2025 Oct 2;

The VCPKMT-VCP-HDAC1 axis inhibits colorectal cancer by conferring sensitivity to ferroptosis.

Huang C, Lin J, Xu S, Lv Y, Chen J, Cao J, Xie D, Wang F
Cancer cell international 2025 Nov 14;25(1):404

Tyrosine and Phenylalanine Activate Neuronal DNA Repair but Exhibit Opposing Effects on Global Transcription and Adult Female Mice Are Resilient to TyrRS/YARS1 Depletion.

Jhanji M, Bhan A, Arrowood C, Yakout DW, Shroff A, McManus D, Gifford H, Vacharasin J, Lizarraga SB, Nazarko TY, Mabb AM, Sajish M
IUBMB life 2025 Jun;77(6):e70030

Selenoprotein K Is a Peripheral ER Membrane Protein.

Odunsi A, Rahmani E, Ghelichkhani F, Romero B, Batish M, Rozovsky S
Biochemistry 2025 Jun 17;64(12):2649-2659

Proteostasis and autophagy disruption by the aging-related VGVAPG hexapeptide - preliminary insights into a potential novel elastin-induced neurodegeneration pathway in an in vitro human cellular neuron model.

Skóra B, Szychowski KA
Neurochemistry international 2025 Jul;187:105992

Trim21 modulates endoplasmic reticulum-associated degradation and sensitizes cancer cells to ER stress-induced apoptosis by inhibiting VCP/Npl4/UFD1 assembly.

Yuan C, Liao Y, Si W, Huang M, Li D, Wang F, Quan Y, Yu X, Liao S
Biochimica et biophysica acta. Molecular basis of disease 2025 Jan;1871(1):167533

Targeting EGFR-binding protein SLC7A11 enhancing antitumor immunity of T cells via inducing MHC-I antigen presentation in nasopharyngeal carcinoma.

Wang H, Fan S, Zhan Y, Xu Y, Du Y, Luo J, Zang H, Peng S, Wang W
Cell death & disease 2025 Jan 16;16(1):21

Naringenin inhibits ferroptosis to reduce radiation-induced lung injury: insights from network Pharmacology and molecular docking.

Jiang J, Deng X, Xu C, Wu Y, Huang J
Pharmaceutical biology 2025 Dec;63(1):1-10

Diet-induced RKIP downregulation disrupts PC/PE-ER homeostasis to drive MASLD.

Li M, Ou Q, Qin Q, Chen J, Yang S, Zhao J, Meng H, Li X, Xu P, Ye C, Wang X
Nature communications 2025 Dec 12;16(1):11092

Response of UBR-box E3 ubiquitin ligases and protein quality control pathways to perturbations in protein synthesis and skeletal muscle size.

Baehr LM, Oliveira de Sousa LG, Goodman CA, Sharples AP, Waddell DS, Bodine SC, Hughes DC
American journal of physiology. Cell physiology 2025 Dec 1;329(6):C1706-C1722

Episodic transport of protein aggregates achieves a positive size selectivity in aggresome formation.

Fang R, Bai L, Verheijen BM, Li B, Dong K, Paulo JA, Zhou M, Chu YC, Song Y, Sherman MY, Gygi S, Field CM, Mitchison TJ, Lu Y
Nature communications 2025 Aug 22;16(1):7852

The p97-UBXD8 complex maintains peroxisome abundance by suppressing pexophagy.

Montes ID, Amirthagunanathan S, Joshi AS, Raman M
bioRxiv : the preprint server for biology 2024 Sep 26;

TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival.

Lascaux P, Hoslett G, Tribble S, Trugenberger C, Antičević I, Otten C, Torrecilla I, Koukouravas S, Zhao Y, Yang H, Aljarbou F, Ruggiano A, Song W, Peron C, Deangeli G, Domingo E, Bancroft J, Carrique L, Johnson E, Vendrell I, Fischer R, Ng AWT, Ngeow J, D'Angiolella V, Raimundo N, Maughan T, Popović M, Milošević I, Ramadan K
Cell 2024 Oct 3;187(20):5698-5718.e26

Upregulation of ENAH by a PI3K/AKT/β-catenin cascade promotes oral cancer cell migration and growth via an ITGB5/Src axis.

Chan XY, Chang KP, Yang CY, Liu CR, Hung CM, Huang CC, Liu HP, Wu CC
Cellular & molecular biology letters 2024 Nov 7;29(1):136

HNF-1β alleviates podocyte injury in lupus nephritis by maintaining endoplasmic reticulum homeostasis.

Zou HM, Yu J, Ruan YY, Xie Y, An XM, Chen PL, Luo YQ, Shi MJ, Liu M, Xu LF, Liu J, Guo B, Zhang F
Lupus science & medicine 2024 Nov 27;11(2)

Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats.

Tseng YJ, Krans A, Malik I, Deng X, Yildirim E, Ovunc S, Tank EMH, Jansen-West K, Kaufhold R, Gomez NB, Sher R, Petrucelli L, Barmada SJ, Todd PK
Nucleic acids research 2024 Jun 10;52(10):5928-5949

New mode of action of curcumin on prostate cancer cells: Modulation of endoplasmic reticulum-associated degradation mechanism and estrogenic signaling.

Erzurumlu Y, Dogan HK, Catakli D
Journal of biochemical and molecular toxicology 2024 Jan;38(1):e23636

Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders.

Wang HH, Lin LL, Li ZJ, Wei X, Askander O, Cappuccio G, Hashem MO, Hubert L, Munnich A, Alqahtani M, Pang Q, Burmeister M, Lu Y, Poirier K, Besmond C, Sun S, Brunetti-Pierri N, Alkuraya FS, Qi L
The Journal of clinical investigation 2024 Jan 16;134(2)

UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation.

Ahlstedt BA, Ganji R, Mukkavalli S, Paulo JA, Gygi SP, Raman M
EMBO reports 2024 Feb;25(2):672-703

XAF1 promotes colorectal cancer metastasis via VCP-RNF114-JUP axis.

Xia J, Ma N, Shi Q, Liu QC, Zhang W, Cao HJ, Wang YK, Zheng QW, Ni QZ, Xu S, Zhu B, Qiu XS, Ding K, Huang JY, Liang X, Chen Y, Xiang YJ, Zhang XR, Qiu L, Chen W, Xie D, Wang X, Long L, Li JJ
The Journal of cell biology 2024 Feb 5;223(2)

hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility.

Wang X, Fan X, Zhang J, Wang F, Chen J, Wen Y, Wang L, Li T, Li H, Gu H, Zhang Y, Yuan S
Cell reports 2024 Feb 27;43(2):113769

SEL1L-HRD1 interaction is required to form a functional HRD1 ERAD complex.

Lin LL, Wang HH, Pederson B, Wei X, Torres M, Lu Y, Li ZJ, Liu X, Mao H, Wang H, Zhou LE, Zhao Z, Sun S, Qi L
Nature communications 2024 Feb 16;15(1):1440

VCP/p97-associated proteins are binders and debranching enzymes of K48-K63-branched ubiquitin chains.

Lange SM, McFarland MR, Lamoliatte F, Carroll T, Krshnan L, Pérez-Ràfols A, Kwasna D, Shen L, Wallace I, Cole I, Armstrong LA, Knebel A, Johnson C, De Cesare V, Kulathu Y
Nature structural & molecular biology 2024 Dec;31(12):1872-1887

Stool Protein Mass Spectrometry Identifies Biomarkers for Early Detection of Diffuse-type Gastric Cancer.

Ho CC, Gilbert MB, Urtecho G, Lee H, Drew DA, Klempner SJ, Cho JS, Ryan TJ, Rustgi N, Lee H, Lee J, Caraballo A, Magicheva-Gupta MV, Rios C, Shin AE, Tseng YY, Davis JL, Chung DC, Chan AT, Wang HH, Ryeom S
Cancer prevention research (Philadelphia, Pa.) 2024 Aug 1;17(8):361-376

Circadian Oscillation Pattern of Endoplasmic Reticulum Quality Control (ERQC) Components in Human Embryonic Kidney HEK293 Cells.

Erzurumlu Y, Catakli D, Dogan HK
Journal of circadian rhythms 2023;21:1

Estrogens drive the endoplasmic reticulum-associated degradation and promote proto-oncogene c-Myc expression in prostate cancer cells by androgen receptor/estrogen receptor signaling.

Erzurumlu Y, Dogan HK, Catakli D, Aydogdu E, Muhammed MT
Journal of cell communication and signaling 2023 Sep;17(3):793-811

Triiodothyronine positively regulates endoplasmic reticulum-associated degradation (ERAD) and promotes androgenic signaling in androgen-dependent prostate cancer cells.

Erzurumlu Y, Muhammed MT
Cellular signalling 2023 Sep;109:110745

G3BP1 coordinates lysophagy activity to protect against compression-induced cell ferroptosis during intervertebral disc degeneration.

Li S, Liao Z, Yin H, Liu O, Hua W, Wu X, Zhang Y, Gao Y, Yang C
Cell proliferation 2023 Mar;56(3):e13368

1,25(OH)(2) D(3) induced vitamin D receptor signaling negatively regulates endoplasmic reticulum-associated degradation (ERAD) and androgen receptor signaling in human prostate cancer cells.

Erzurumlu Y, Aydogdu E, Dogan HK, Catakli D, Muhammed MT, Buyuksandic B
Cellular signalling 2023 Mar;103:110577

GLI1, a novel target of the ER stress regulator p97/VCP, promotes ATF6f-mediated activation of XBP1.

Almada LL, Barroso K, Sen S, Toruner M, Sigafoos AN, Raja Arul GL, Pease DR, Vera RE, Olson RLO, Auner HW, Pedeux R, Iovanna JL, Chevet E, Fernandez-Zapico ME
Biochimica et biophysica acta. Gene regulatory mechanisms 2023 Jun;1866(2):194924

Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats.

Tseng YJ, Malik I, Deng X, Krans A, Jansen-West K, Tank EMH, Gomez NB, Sher R, Petrucelli L, Barmada SJ, Todd PK
bioRxiv : the preprint server for biology 2023 Jun 7;

CircHIPK3 negatively regulates autophagy by blocking VCP binding to the Beclin 1 complex in bladder cancer.

Wang C, Liu T, Wang J, Cheng C, Zhang Z, Zhang J, Huang H, Li Y
Discover oncology 2023 Jun 3;14(1):86

Dexamethasone-stimulated glucocorticoid receptor signaling positively regulates the endoplasmic reticulum-associated degradation (ERAD) mechanism in hepatocellular carcinoma cells.

Erzurumlu Y, Dogan HK, Cataklı D
Steroids 2023 Jul;195:109238

Progesterone regulates the endoplasmic reticulum-associated degradation and Unfolded Protein Response axis by mimicking the androgenic stimulation in prostate cancer cells.

Erzurumlu Y, Dogan HK, Catakli D
Molecular biology reports 2023 Feb;50(2):1253-1265

The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition.

Ganji R, Paulo JA, Xi Y, Kline I, Zhu J, Clemen CS, Weihl CC, Purdy JG, Gygi SP, Raman M
Nature communications 2023 Feb 6;14(1):638

Inhibition of VCP modulates NF-κB signaling pathway to suppress multiple myeloma cell proliferation and osteoclast differentiation.

Wei R, Cao Y, Wu H, Liu X, Jiang M, Luo X, Deng Z, Wang Z, Ke M, Zhu Y, Chen S, Gu C, Yang Y
Aging 2023 Aug 21;15(16):8220-8236

Valosin-containing protein (VCP/p97) is responsible for the endocytotic trafficking of classical swine fever virus.

Sun RC, Hu JH, Li XH, Liu CC, Liu YY, Chen J, Yang YC, Zhou B
Veterinary microbiology 2022 Sep;272:109511

UBXD8 mediates mitochondria-associated degradation to restrain apoptosis and mitophagy.

Zheng J, Cao Y, Yang J, Jiang H
EMBO reports 2022 Oct 6;23(10):e54859

An eIF3d-dependent switch regulates HCMV replication by remodeling the infected cell translation landscape to mimic chronic ER stress.

Thompson L, Depledge DP, Burgess HM, Mohr I
Cell reports 2022 May 3;39(5):110767

Targeting non-canonical pathways as a strategy to modulate the sodium iodide symporter.

Read ML, Brookes K, Thornton CEM, Fletcher A, Nieto HR, Alshahrani M, Khan R, Borges de Souza P, Zha L, Webster JRM, Alderwick LJ, Campbell MJ, Boelaert K, Smith VE, McCabe CJ
Cell chemical biology 2022 Mar 17;29(3):502-516.e7

The Mechano-Ubiquitinome of Articular Cartilage: Differential Ubiquitination and Activation of a Group of ER-Associated DUBs and ER Stress Regulators.

Kaokhum N, Pinto-Fernández A, Wilkinson M, Kessler BM, Ismail HM
Molecular & cellular proteomics : MCP 2022 Dec;21(12):100419

MEST promotes lung cancer invasion and metastasis by interacting with VCP to activate NF-κB signaling.

Wang Y, Zhang J, Li YJ, Yu NN, Liu WT, Liang JZ, Xu WW, Sun ZH, Li B, He QY
Journal of experimental & clinical cancer research : CR 2021 Sep 24;40(1):301

SAA1 is transcriptionally activated by STAT3 and accelerates renal interstitial fibrosis by inducing endoplasmic reticulum stress.

Zhang F, Zhou X, Zou H, Liu L, Li X, Ruan Y, Xie Y, Shi M, Xiao Y, Wang Y, Zhou Y, Wu Y, Guo B
Experimental cell research 2021 Nov 1;408(1):112856

p97/VCP inhibition causes excessive MRE11-dependent DNA end resection promoting cell killing after ionizing radiation.

Kilgas S, Singh AN, Paillas S, Then CK, Torrecilla I, Nicholson J, Browning L, Vendrell I, Konietzny R, Kessler BM, Kiltie AE, Ramadan K
Cell reports 2021 May 25;35(8):109153

Analysis of Zika virus capsid-Aedes aegypti mosquito interactome reveals pro-viral host factors critical for establishing infection.

Gestuveo RJ, Royle J, Donald CL, Lamont DJ, Hutchinson EC, Merits A, Kohl A, Varjak M
Nature communications 2021 May 13;12(1):2766

Hyaluronic acid/polyethyleneimine nanoparticles loaded with copper ion and disulfiram for esophageal cancer.

Xu R, Zhang K, Liang J, Gao F, Li J, Guan F
Carbohydrate polymers 2021 Jun 1;261:117846

SPRTN protease-cleaved MRE11 decreases DNA repair and radiosensitises cancer cells.

Na J, Newman JA, Then CK, Syed J, Vendrell I, Torrecilla I, Ellermann S, Ramadan K, Fischer R, Kiltie AE
Cell death & disease 2021 Feb 8;12(2):165

The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability.

Ruggiano A, Vaz B, Kilgas S, Popović M, Rodriguez-Berriguete G, Singh AN, Higgins GS, Kiltie AE, Ramadan K
Cell reports 2021 Dec 7;37(10):110080

Berberine alleviates liver fibrosis through inducing ferrous redox to activate ROS-mediated hepatic stellate cells ferroptosis.

Yi J, Wu S, Tan S, Qin Y, Wang X, Jiang J, Liu H, Wu B
Cell death discovery 2021 Dec 4;7(1):374

VPS13D interacts with VCP/p97 and negatively regulates endoplasmic reticulum-mitochondria interactions.

Du Y, Wang J, Xiong J, Fang N, Ji WK
Molecular biology of the cell 2021 Aug 1;32(16):1474-1486

Vitamin D Attenuates Alzheimer-like Pathology Induced by Okadaic Acid.

Pan Y, Zhang Y, Liu N, Lu W, Yang J, Li Y, Liu Z, Wei Y, Lou Y, Kong J
ACS chemical neuroscience 2021 Apr 21;12(8):1343-1350

The p97-UBXN1 complex regulates aggresome formation.

Mukkavalli S, Klickstein JA, Ortiz B, Juo P, Raman M
Journal of cell science 2021 Apr 1;134(7)

Active Protein Neddylation or Ubiquitylation Is Dispensable for Stress Granule Dynamics.

Markmiller S, Fulzele A, Higgins R, Leonard M, Yeo GW, Bennett EJ
Cell reports 2019 Apr 30;27(5):1356-1363.e3

The VCP-UBXN1 Complex Mediates Triage of Ubiquitylated Cytosolic Proteins Bound to the BAG6 Complex.

Ganji R, Mukkavalli S, Somanji F, Raman M
Molecular and cellular biology 2018 Jul 1;38(13)

AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.

Luo H, Jiang M, Lian G, Liu Q, Shi M, Li TY, Song L, Ye J, He Y, Yao L, Zhang C, Lin ZZ, Zhang CS, Zhao TJ, Jia WP, Li P, Lin SY, Lin SC
Cell metabolism 2018 Apr 3;27(4):843-853.e6

Acupuncture regulates the unfolded protein response and inhibits apoptosis in a rat model of heroin relapse.

Zhang Y, Cai XH, Zhang RJ, Hou XR, Song XG, Wu SB, Yu S, Cao JP
Acupuncture in medicine : journal of the British Medical Acupuncture Society 2016 Dec;34(6):441-448

Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.

Tao Z, Wang H, Xia Q, Li K, Li K, Jiang X, Xu G, Wang G, Ying Z
Human molecular genetics 2015 May 1;24(9):2426-41

Integrative quantitative proteomics unveils proteostasis imbalance in human hepatocellular carcinoma developed on nonfibrotic livers.

Negroni L, Taouji S, Arma D, Pallares-Lupon N, Leong K, Beausang LA, Latterich M, Bossé R, Balabaud C, Schmitter JM, Bioulac-Sage P, Zucman-Rossi J, Rosenbaum J, Chevet E
Molecular & cellular proteomics : MCP 2014 Dec;13(12):3473-83

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10736-1-AP(VCP antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-VCP (IP:10736-1-AP, 4ug; Detection:10736-1-AP 1:1000) with HeLa cells lysate 1505ug.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human gliomas using 10736-1-AP(VCP antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The VCP antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human VCP. This antibody recognizes human,mouse,rat antigen. The VCP antibody has been validated for the following applications: ELISA, WB, IP, IHC analysis.
Appendix: 10736-1-AP.pdf

: Show more