Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [3]
- Immunohistochemistry [12]
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- Product number
- LS-C313248 - Provider product page
- Provider
- LSBio
- Product name
- VCP Antibody (aa749-766) LS-C313248
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Drosophila, Guinea Pig, Horse, Porcine, Rabbit, Sheep, Simian, Xenopus, Zebrafish
- Host
- Rabbit
- Storage
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ICC analysis of VCP using anti-VCP antibody. VCP was detected in immunocytochemical section of K562 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-VCP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ICC analysis of VCP using anti-VCP antibody. VCP was detected in immunocytochemical section of K562 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-VCP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ICC analysis of VCP using anti-VCP antibody. VCP was detected in immunocytochemical section of K562 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-VCP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- VCP antibody. IHC(P): Rat Intestine Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- VCP antibody. IHC(P): Human Breast Cancer Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- VCP antibody. IHC(P): Rat Cerebellum Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- VCP antibody. IHC(F): Rat Intestine Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC analysis of VCP using anti-VCP antibody. VCP was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-VCP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- VCP antibody. IHC(P): Rat Epinephros Tissue.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- VCP antibody. IHC(P): Human Breast Cancer Tissue.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- VCP antibody. IHC(F): Rat Intestine Tissue.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- VCP antibody. IHC(P): Rat Intestine Tissue.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- VCP antibody. IHC(P): Rat Cerebellum Tissue.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC analysis of VCP using anti-VCP antibody. VCP was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-VCP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- VCP antibody. IHC(P): Human Breast Cancer Tissue.