SM5062

antibody from Acris Antibodies GmbH

Targeting: VCP CDC48, IBMPFD, p97, TERA

Immunoprecipitation

Flow cytometry

Antibody data

Submit

Validation data

Product number: SM5062 - Provider product page
Provider: Acris Antibodies GmbH
Proper citation: Acris Antibodies GmbH Cat#SM5062, RRID:AB_1008429
Product name: anti TER ATPase / VCP
Antibody type: Monoclonal
Antigen: Synthetic Peptide: C G(792) G S V Y T E D N D D D L Y G (806) corresponding to amino acid residues 792-806 from Mouse VCP.
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: 5
Vial size: 0.1 ml

VCP protein structure - SM5062 shown in red.

Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Immunofluorescent analysis of VCP using VCP Monoclonal antibody (5) (<em>Cat.-No</em> SM5062) shows staining in ViDr colon carcinoma cells. Â F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing VCP (<em>Cat.-No</em> SM5062) at a 1/20-1/200 overnight at 4Â°C, washed with PBS and incubated with a Dylight-488 conjugated secondary antibody.

Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Immunofluorescent analysis of VCP using VCP Monoclonal antibody (5) (Cat.-NoÂ SM5062) shows staining in Hela cells. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing VCP (Cat.-No SM5062) at a 1/20-1/200 overnight at 4Â°C, washed with PBS and incubated with a Dylight-488 conjugated secondary antibody.

Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Immunofluorescent analysis of VCP using VCP Monoclonal antibody (5) (Cat.-NoÂ SM5062) shows staining in C6 glioma cells. VCP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing VCP (Cat.-No SM5062) at a 1/20-1/200 overnight at 4Â°C, washed with PBS and incubated with a Dylight-488 conjugated secondary antibody.

Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human brain tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/1000 with a mouse monoclonal antibody recognizing Anti-VCP (Cat.-No SM5062) or without primary antibody (negative control) overnight at 4Â°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/1000 with SM5062 or with primary antibody (Negative Control) overnight at 4Â°C. in humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.