R31254

antibody from NSJ Bioreagents

Targeting: VCP CDC48, IBMPFD, p97, TERA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R31254

Provider

NSJ Bioreagents

Product name

VCP Antibody

Antibody type

Polyclonal

Description

This highly specific VCP antibody is suitable for use in Western blot/Immunohistochemistry/Immunohistochemistry/Immunocytochemistry/Immunofluorescence/Flow cytometry applications with human, mouse and rat, monkey samples.

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the VCP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of VCP antibody and rat samples 1: brain; 2: kidney; 3: liver; 4: lung; and human samples 5: HeLa; 6: HL-60; 7: A431; 8: A549; 9: SMMC-7721 cell lysate. Expected/observed size ~89KD

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of VCP antibody and rat samples 1: brain; 2: kidney; 3: liver; 4: lung; and human samples 5: HeLa; 6: HL-60; 7: A431; 8: A549; 9: SMMC-7721 cell lysate. Expected/observed size ~89KD

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HeLa, 2) human SH-SY5Y, 3) human MCF7, 4) human A431, 5) human K562, 6) human HepG2 and 7) monkey COS-7 cell lysate with VCP antibody. Predicted molecular weight ~89 kDa, commonly observed at 89-97 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat brain, 2) rat lung, 3) rat stomach, 4) rat C6, 5) mouse brain, 6) mouse lung and 7) mouse RAW264.7 cell lysate with VCP antibody. Predicted molecular weight ~89 kDa, commonly observed at 89-97 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunocytochemical staining of FFPE human K562 cells with VCP antibody. HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human A549 cells with VCP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: VCP antibody testing of human breast cancer tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-F testing of VCP antibody and rat intestine tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: VCP antibody testing of rat intestine tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: VCP antibody testing of rat epinephros tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: VCP antibody testing of rat cerebellum tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-F testing of VCP antibody and rat intestine tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: VCP antibody testing of rat intestine tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: VCP antibody testing of rat epinephros tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: VCP antibody testing of rat cerebellum tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: VCP antibody testing of mouse intestine tissue

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HeLa cells with VCP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VCP antibody.