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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- F52449 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- VCP Antibody
- Antibody type
- Monoclonal
- Antigen
- This VCP antibody was produced from a mouse immunized with a recombinant protein from human VCP.
- Description
- Purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 1344CT150.163.114
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the VCP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from 1) MCF-7, 2) U251, 3) mouse NIH3T3, 4) rat C6 cell line and 5) mouse liver tissue using VCP antibody at 1:1000.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent image of U251 cells stained with VCP antibody at 1:25. An Alexa Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
