Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-29638 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VCP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3 , JC, BCL-1, Rat brain. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (98%), Xenopus laevis (98%), Pig (99%), Chicken (100%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VCP using Various whole cell extracts (30 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a VCP polyclonal antibody (Product # PA5-29638) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VCP using 30 µg of A) NT2D1 (B) U87-MG and C) MCF-7 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a VCP polyclonal antibody (Product # PA5-29638) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of VCP was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a VCP Polyclonal Antibody (Product # PA5-29638) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using VCP Polyclonal Antibody (Product # PA5-29638). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC. Lane C: BCL-1. 7.5% SDS PAGE. VCP Polyclonal Antibody (Product # PA5-29638) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- VCP Polyclonal Antibody detects VCP protein by western blot analysis. A. 50 µg Rat brain lysate/extract.7.5% SDS-PAGE. VCP Polyclonal Antibody (Product # PA5-29638) dilution: 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of VCP was achieved by transfecting MCF7 cells with VCP specific siRNAs (Silencer® select Product # s14765). Western blot analysis (Fig. a) was performed using whole cell extracts from the VCP knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with VCP Polyclonal Antibody (Product # PA5-29638, 1:8000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to VCP.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extract (30 µg lysate) of SKOV3 (Lane 1), MDA-MB-231 (Lane 2), MCF7 (Lane 3), GTL16 (Lane 4), HCT116 (Lane 5), PC3 (Lane 6), A431 (Lane 7), PANC-1 (Lane 8), and A549 (Lane 9). The blot was probed with Anti-VCP Polyclonal Antibody (Product # PA5-29638, 1:8000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 97 kDa band corresponding to VCP was observed in all cell lines.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of VCP in paraformaldehyde-fixed MCF-7 cells using a VCP polyclonal antibody (Product # PA5-29638) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- VCP Polyclonal Antibody detects VCP protein at nucleus on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. VCP Polyclonal Antibody (Product # PA5-29638) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.