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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB100-1557 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1557, RRID:AB_2272824
- Product name
- Rabbit Polyclonal p97/VCP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Alcohol-abuse drug disulfiram targets cancer via p97 segregase adaptor NPL4.
A ubiquitin independent degradation pathway utilized by a hepatitis B virus envelope protein to limit antigen presentation.
Regulation of endoplasmic reticulum-associated degradation by RNF5-dependent ubiquitination of JNK-associated membrane protein (JAMP).
ERdj4 and ERdj5 are required for endoplasmic reticulum-associated protein degradation of misfolded surfactant protein C.
Skrott Z, Mistrik M, Andersen KK, Friis S, Majera D, Gursky J, Ozdian T, Bartkova J, Turi Z, Moudry P, Kraus M, Michalova M, Vaclavkova J, Dzubak P, Vrobel I, Pouckova P, Sedlacek J, Miklovicova A, Kutt A, Li J, Mattova J, Driessen C, Dou QP, Olsen J, Hajduch M, Cvek B, Deshaies RJ, Bartek J
Nature 2017 Dec 14;552(7684):194-199
Nature 2017 Dec 14;552(7684):194-199
A ubiquitin independent degradation pathway utilized by a hepatitis B virus envelope protein to limit antigen presentation.
Liu Y, Testa JS, Philip R, Block TM, Mehta AS
PloS one 2011;6(9):e24477
PloS one 2011;6(9):e24477
Regulation of endoplasmic reticulum-associated degradation by RNF5-dependent ubiquitination of JNK-associated membrane protein (JAMP).
Tcherpakov M, Delaunay A, Toth J, Kadoya T, Petroski MD, Ronai ZA
The Journal of biological chemistry 2009 May 1;284(18):12099-109
The Journal of biological chemistry 2009 May 1;284(18):12099-109
ERdj4 and ERdj5 are required for endoplasmic reticulum-associated protein degradation of misfolded surfactant protein C.
Dong M, Bridges JP, Apsley K, Xu Y, Weaver TE
Molecular biology of the cell 2008 Jun;19(6):2620-30
Molecular biology of the cell 2008 Jun;19(6):2620-30
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p97/VCP Antibody [NB100-1557] - Detection of Human and Mouse VCP on HeLa whole cell lysate using NB100-1557. VCP was also immunoprecipitated using rabbit anti-VCP antibodies NB100-1558 and Another VCP Ab at 3 mcg/mg lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p97/VCP Antibody [NB100-1557] - PC12 whole cell lysate, 30 ug total protein in each lane. Antibody at 1:1000. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: p97/VCP Antibody [NB100-1557] - Sample: FFPE sections of human ovarian carcinoma (upper) and mouse squamous cell carcinoma (lower). Antibody: Affinity purified rabbit anti-VCP used at a dilution of 1:200 (1ug/ml). Detection: DAB
