HPA012728

antibody from Atlas Antibodies

Targeting: VCP CDC48, IBMPFD, p97, TERA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA012728

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA012728, RRID:AB_1858730

Product name

Anti-VCP

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

LSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCP
DVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLE
AYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIV
APDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRK
QLAQIKEMVE


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VCP antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line A-549.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human hippocampus shows strong nuclear and cytoplasmic positivity in neuronal cells and glial cells.

Sample type

HUMAN