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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21962 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAB2B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: HepG2, mouse brain. Predicted reactivity: Mouse (94%), Rat (95%), Xenopus laevis (87%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.83 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAB2B using 50 µg of mouse brain lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a RAB2B polyclonal antibody (Product # PA5-21962) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RAB2B Polyclonal Antibody (Product # PA5-21962). Sample (30 µg whole cell lysate). A: Hep G2. 12% SDS PAGE. RAB2B Polyclonal Antibody (Product # PA5-21962) diluted at 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RAB2B Polyclonal Antibody (Product # PA5-21962). Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB2B Polyclonal Antibody (Product # PA5-21962) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RAB2B in paraformaldehyde-fixed HeLa cells using a RAB2B polyclonal antibody (Product # PA5-21962) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAB2B (Product # PA5-21962) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RAB2B Polyclonal Antibody detects RAB2B protein at cytoplasm and membrane on human liver carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded liver carcinoma. RAB2B Polyclonal Antibody (Product # PA5-21962) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
