Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF6589 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Desmoglein-4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Desmoglein-4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Desmoglein-1, rhDesmoglein-2, and rhDesmoglein-3 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q86SJ6
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references An autosomal recessive mutation of DSG4 causes monilethrix through the ER stress response.
Kato M, Shimizu A, Yokoyama Y, Kaira K, Shimomura Y, Ishida-Yamamoto A, Kamei K, Tokunaga F, Ishikawa O
The Journal of investigative dermatology 2015 May;135(5):1253-1260
The Journal of investigative dermatology 2015 May;135(5):1253-1260
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human Desmoglein-4 by Western Blot. Western blot shows lysates of human skin tissue. PVDF Membrane was probed with 0.1 µg/mL of Human Desmoglein-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6589) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Desmoglein-4 at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.