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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00002580-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002580-M01, RRID:AB_539035
- Product name
- Mouse Monoclonal GAK Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. GAK - cyclin G associated kinase
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAK Antibody (4C10) [H00002580-M01] - Analysis of GAK expression in transfected 293T cell line by GAK monoclonal antibody (M01), clone 4C10.Lane 1: GAK transfected lysate (Predicted MW: 44 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAK Antibody (4C10) [H00002580-M01] - GAK monoclonal antibody (M01), clone 4C10 Analysis of GAK expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: GAK Antibody (4C10) [H00002580-M01] - Detection limit for recombinant GST tagged GAK is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: GAK Antibody (4C10) [H00002580-M01] - Analysis of GAK transfected lysate using anti-GAK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GAK monoclonal antibody.
