PA5-89087

antibody from Invitrogen Antibodies

Targeting: RBM11

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-89087

Provider

Invitrogen Antibodies

Product name

RBM11 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MFPAQEEADR TVFVGNLEAR VREEILYELF LQAGPLTKVT ICKDREGKPK SFGFVCFKHP ESVSYAIALL NGIRLYGRPI NVQYRFGSSR SSEPANQSFE SCVKINSHNY RNEEMLVGRS SFPMQYFPIN NTSLPQEYFL FQKMQWHVYN PVLQLPYYEM TAPLPNSASV SSSLNHVPDL EAGPSSYKWT HQQPSDSDLY QMTAPLPNSA SVSSSLNHVP DLEAGPSSYK WTHQQPSDSD LYQMNKRKRQ KQTSDSDSST DNNRGNECSQ KFRKSKKKKR Y; Positive Samples: U-87MG, 293T, MCF7, Mouse testis, Rat testis; Cellular Location: Nucleus, Nucleus speckle, nucleoplasm

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.58 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using RBM11 Polyclonal antibody (Product # PA5-89087) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RBM11 in extracts of various cell lines using RBM11 Polyclonal Antibody (Product # PA5-89087) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.